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| Status |
Public on Sep 01, 2013 |
| Title |
Identification of a microRNA expression signature for radiochemosensitivity of colorectal cancer cells, involving miRNAs-320a, -224, -132 and let7g |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Purpose: Preoperative 5-fluorouracil (5-FU) based radiochemotherapy (RCT) represents the standard treatment for locally advanced rectal cancer. Both, tumor response and progression vary considerably. MicroRNAs represent master regulators of gene expression, and may therefore contribute to this heterogeneity.
Results: Thirty-six miRNAs were identified to significantly correlate with sensitivity of CRC cell lines to RCT (q < 0.05). This list included miR320a as most significantly correlated as well as other miRNAs involved in the MAPK-, TGF- and Wnt-pathway. Importantly, transfection of selected miRNAs (let7g, miR-132, miR-224, miR-320a and miR-429) each induced a shift of sensitivity (p<0.00001). Moreover, high expression of miR-224 was associated with a poor prognosis in rectal cancer patients (p=0.043).
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| Overall design |
Experimental design: Genome-wide microRNA (miRNA) profiling was performed from 12 colorectal cancer (CRC) cell lines. To establish an in vitro signature of radiochemosensitivity, these profiles were correlated to the individual sensitivities of each cell line to 5-FU and radiation. The functional relevance of selected miRNAs was validated by transfecting miRNA-mimics into SW480 cells, followed by treatment with 5-FU and radiation. 12 Samples with 3 replicates each.
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| Contributor(s) |
Salendo J, Spitzner M, Kramer F, Zhang X, Emons G, Jo P, Wolff HA, Kitz J, Kaulfuß S, Beißbarth T, Dobbelstein M, Ghadimi M, Grade M, Gaedcke J |
| Citation(s) |
23932154 |
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| Submission date |
Sep 18, 2012 |
| Last update date |
Jun 07, 2015 |
| Contact name |
Frank Kramer |
| E-mail(s) |
frank.kramer@med.uni-goettingen.de
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| Organization name |
University Medical Center Göttingen
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| Department |
Department of Medical Statistics
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| Street address |
Humboldtallee 32
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| City |
Goettingen |
| ZIP/Postal code |
37073 |
| Country |
Germany |
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| Platforms (1) |
| GPL16770 |
Agilent-031181 Unrestricted_Human_miRNA_V16.0_Microarray (miRBase release 16.0 miRNA ID version) |
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| Samples (35)
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| Relations |
| BioProject |
PRJNA175468 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE40976_RAW.tar |
105.0 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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