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Status |
Public on Nov 29, 2012 |
Title |
Coral thermal tolerance: tuning gene expression to resist thermal stress |
Organism |
Acropora millepora |
Experiment type |
Expression profiling by array
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Summary |
Thermal history plays a role in the response of corals to subsequent heat stress. Prior heat stress can have a profound impact on later thermal tolerance, but the mechanism for this plasticity is not clear. The understanding of gene expression changes behind physiological acclimatization is critical in forecasts of coral health in impending climate change scenarios. Acropora millepora fragments were preconditioned to sublethal bleaching threshold stress for a period of 10 days; this prestress conferred bleaching resistance in subsequent thermal challenge, in which non-preconditioned coral bleached. Using microarrays, we analyze the transcriptomes of the coral host, comparing the bleaching-resistant preconditioned treatment to non-preconditioned and control treatments.
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Overall design |
This experiment compared host gene expression of Acropora millepora across control, non-preconditioned, and preconditioned treatments. Fragments were sampled prior to preconditioning (Day 4), following 10 days of thermal preconditioning (Day 20), and after two (Day 23), four (Day 25), and eight days (Day 29) of 31°C thermal challenge. The analysis implements 45 arrays, representing 5 sampling points of three treatments (n=3).
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Contributor(s) |
Bellantuono AJ, Rodriguez-Lanetty M |
Citation(s) |
23226355 |
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Submission date |
Oct 09, 2012 |
Last update date |
Feb 28, 2013 |
Contact name |
Anthony John Bellantuono |
Organization name |
Florida International University
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Department |
Biological Sciences
|
Lab |
Rodriguez-Lanetty
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Street address |
11200 SW 8th
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City |
Miami |
State/province |
FL |
ZIP/Postal code |
33199 |
Country |
USA |
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Platforms (1) |
GPL6941 |
Australian National University Acropora millepora 18k array version 1 |
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Samples (45)
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Relations |
BioProject |
PRJNA176944 |