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| Status |
Public on Oct 15, 2013 |
| Title |
Taqman Low Density Arrays (TLDA) based microRNAs expression profile of mouse extrahepatic bileducts and gallbladders during a mouse model of biliary atresia. |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
Newborn Balb/c mice were injected with 1.5x10^6 fluorescent-forming units (ffu) of Rhesus rotavirus type-A or 0.9% NaCl (normal saline) intraperitoneally within 24 hours of birth to induce experimental model of biliary atresia. The extrahepatic bile ducts including gallbladder were microdissected en bloc at 3, 7 and 14 days after rhesus rotavirus or saline injection. TaqMan® Array Rodent MicroRNA Card v2.0 (A and B) were used to screen microRNAs whose expression was differently regulated after rhusus rotavirus injection compare to the normal saline controls.
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| Overall design |
microRNA expression profiling. Each experimental conditon has 3 sets samples . Two to six extrahepatic bileducts were pooled prior to total RNA isolation depending on the size to ensure adequate RNA quantities to perform experiments quantifying microRNA expression.
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| Contributor(s) |
Bessho K, Shanmukhappa K, Sheridan R, Shivakumar P, Mourya R, Walters S, Kaimal V, Dilbone E, Jegga AG, Bezerra JA |
| Citation(s) |
24138927 |
| |
| Submission date |
Oct 15, 2012 |
| Last update date |
Jan 15, 2014 |
| Contact name |
Jorge A Bezerra |
| E-mail(s) |
jorge.bezerra@cchmc.org
|
| Organization name |
Cincinnati Children's Hospital Medical Center
|
| Department |
Gastroenterology, Hepatology & Nutrition
|
| Street address |
3333 Burnet Ave.
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
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| Platforms (1) |
| GPL15053 |
Applied Biosystems Taqman Array Rodent MicroRNA Cards v2.0 |
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| Samples (18)
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| GSM1019824 |
Extrahepatic Bileduct and gallbladder en bloc at day 3 after normal saline injection miRNA rep1 |
| GSM1019825 |
Extrahepatic Bileduct and gallbladder en bloc at day 3 after normal saline injection miRNA rep2 |
| GSM1019826 |
Extrahepatic Bileduct and gallbladder en bloc at day 3 after normal saline injection miRNA rep3 |
| GSM1019827 |
Extrahepatic Bileduct and gallbladder en bloc at day 3 after rhusus rotvirus challenge miRNA rep1 |
| GSM1019828 |
Extrahepatic Bileduct and gallbladder en bloc at day 3 after rhusus rotvirus challenge miRNA rep2 |
| GSM1019829 |
Extrahepatic Bileduct and gallbladder en bloc at day 3 after rhusus rotvirus challenge miRNA rep3 |
| GSM1019830 |
Extrahepatic Bileduct and gallbladder en bloc at day 7 after normal saline injection miRNA rep1 |
| GSM1019831 |
Extrahepatic Bileduct and gallbladder en bloc at day 7 after normal saline injection miRNA rep2 |
| GSM1019832 |
Extrahepatic Bileduct and gallbladder en bloc at day 7 after normal saline injection miRNA rep3 |
| GSM1019833 |
Extrahepatic Bileduct and gallbladder en bloc at day 7 after rhusus rotvirus challenge miRNA rep1 |
| GSM1019834 |
Extrahepatic Bileduct and gallbladder en bloc at day 7 after rhusus rotvirus challenge miRNA rep2 |
| GSM1019835 |
Extrahepatic Bileduct and gallbladder en bloc at day 7 after rhusus rotvirus challenge miRNA rep3 |
| GSM1019836 |
Extrahepatic Bileduct and gallbladder en bloc at day 14 after normal saline injection miRNA rep1 |
| GSM1019837 |
Extrahepatic Bileduct and gallbladder en bloc at day 14 after normal saline injection miRNA rep2 |
| GSM1019838 |
Extrahepatic Bileduct and gallbladder en bloc at day 14 after normal saline injection miRNA rep3 |
| GSM1019839 |
Extrahepatic Bileduct and gallbladder en bloc at day 14 after rhusus rotvirus challenge miRNA rep1 |
| GSM1019840 |
Extrahepatic Bileduct and gallbladder en bloc at day 14 after rhusus rotvirus challenge miRNA rep2 |
| GSM1019841 |
Extrahepatic Bileduct and gallbladder en bloc at day 14 after rhusus rotvirus challenge miRNA rep3 |
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| This SubSeries is part of SuperSeries: |
| GSE41595 |
Integrative genomics identifies candidate microRNAs for pathogenesis of experimental biliary atresia |
|
| Relations |
| BioProject |
PRJNA177630 |
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