|
| Status |
Public on Nov 15, 2012 |
| Title |
Characterization of the EZH2-MMSET histone methyltransferase regulatory axis in cancer [expression] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Histone methyltransferases (HMTases), as chromatin modifiers, regulate the transcriptomic landscape in normal development as well in diseases such as cancer. Here, we molecularly order two HMTases, EZH2 and MMSET that have established genetic links to oncogenesis. EZH2, which mediates histone H3K27 trimethylation and is associated with gene silencing, was shown to be coordinately expressed and function upstream of MMSET, which mediates H3K36 dimethylation and is associated with active transcription. We found that the EZH2-MMSET HMTase axis is coordinated by a microRNA network and that the oncogenic functions of EZH2 require MMSET activity. Together, these results suggest that the EZH2-MMSET HMTase axis coordinately functions as a master regulator of transcriptional repression, activation, and oncogenesis and may represent an attractive therapeutic target in cancer.
|
| |
|
| Overall design |
LacZ control vs MMSET overexpression or MMSET DeltaSET overexpression in replicates
|
| |
|
| Contributor(s) |
Jing X, Asangani IA |
| Citation(s) |
23159737 |
| |
| Submission date |
Oct 17, 2012 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Meng Zhao |
| E-mail(s) |
meng.zhao@emory.edu
|
| Phone |
404-712-8611
|
| Organization name |
Emory University
|
| Department |
BIOS
|
| Lab |
Steve Qin
|
| Street address |
1518 Clifton Rd, #369
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30322 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
|
| Samples (6)
|
| GSM1020995 |
MMSET overexpression in H16N2 Cell Replicate 1 |
| GSM1020996 |
MMSET overexpression in H16N2 Cell Replicate 2 |
| GSM1020997 |
MMSET overexpression in HME Cell Replicate 1 |
| GSM1020998 |
MMSET overexpression in HME Cell Replicate 2 |
| GSM1020999 |
MMSET DeltSET overexpression in HME Cell Replicate 1 |
| GSM1021000 |
MMSET DeltSET overexpression in HME Cell Replicate 2 |
|
| This SubSeries is part of SuperSeries: |
| GSE41653 |
Characterization of the EZH2-MMSET histone methyltransferase regulatory axis in cancer |
|
| Relations |
| BioProject |
PRJNA177817 |
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