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| Status |
Public on Jun 28, 2013 |
| Title |
Expression changes between loricrin knockout and wildtype P0 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The loss of loricrin, a major component of the cornified envelope, results in a delay of epidermal barrier formation. Therefore, the living layers of the epidermis are aberrantly exposed to late-stage amniotic fluid, which may serve as the signal to upregulate genes that functionally compensate for the loss of loricrin. Consistent with this hypothesis, metabolomic studies revealed marked changes in amniotic fluid between E14.5 and E16.5 dpc. In addition, we discovered that the Nrf2/Keap1 pathway detects these compositional changes and directly upregulates the expression of genes involved in the compensatory response, thus ensuring postnatal survival. In support of this finding, we demonstrate that genetically blocking the Nrf2 pathway abolishes the compensatory response, and preemptively activating Nrf2 pharmacologically rescues the delay in barrier formation in utero. Our findings reveal that the functions of Nrf2 and the composition of amniotic fluid have co-evolved to ensure the formation of a functional barrier.
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| Overall design |
1 sample with one replicate for each genotype was performed for both loricrin knockout and wildtype
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| Contributor(s) |
Roop D |
| Citation(s) |
23237955 |
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| Submission date |
Jun 28, 2013 |
| Last update date |
Feb 18, 2018 |
| Contact name |
Dennis R. Roop |
| E-mail(s) |
dennis.roop@ucdenver.edu
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| Organization name |
University of Colorado
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| Department |
Dermatology
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| Street address |
12800 E. 19th Ave.
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| City |
Aurora |
| State/province |
Colorado |
| ZIP/Postal code |
80045 |
| Country |
USA |
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| Platforms (2) |
| GPL81 |
[MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array |
| GPL82 |
[MG_U74Bv2] Affymetrix Murine Genome U74B Version 2 Array |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA209943 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE41742_RAW.tar |
11.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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