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Series GSE41742 Query DataSets for GSE41742
Status Public on Jun 28, 2013
Title Expression changes between loricrin knockout and wildtype P0
Organism Mus musculus
Experiment type Expression profiling by array
Summary The loss of loricrin, a major component of the cornified envelope, results in a delay of epidermal barrier formation. Therefore, the living layers of the epidermis are aberrantly exposed to late-stage amniotic fluid, which may serve as the signal to upregulate genes that functionally compensate for the loss of loricrin. Consistent with this hypothesis, metabolomic studies revealed marked changes in amniotic fluid between E14.5 and E16.5 dpc. In addition, we discovered that the Nrf2/Keap1 pathway detects these compositional changes and directly upregulates the expression of genes involved in the compensatory response, thus ensuring postnatal survival. In support of this finding, we demonstrate that genetically blocking the Nrf2 pathway abolishes the compensatory response, and preemptively activating Nrf2 pharmacologically rescues the delay in barrier formation in utero. Our findings reveal that the functions of Nrf2 and the composition of amniotic fluid have co-evolved to ensure the formation of a functional barrier.
 
Overall design 1 sample with one replicate for each genotype was performed for both loricrin knockout and wildtype
 
Contributor(s) Roop D
Citation(s) 23237955
Submission date Jun 28, 2013
Last update date Feb 18, 2018
Contact name Dennis R. Roop
E-mail(s) dennis.roop@ucdenver.edu
Organization name University of Colorado
Department Dermatology
Street address 12800 E. 19th Ave.
City Aurora
State/province Colorado
ZIP/Postal code 80045
Country USA
 
Platforms (2)
GPL81 [MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array
GPL82 [MG_U74Bv2] Affymetrix Murine Genome U74B Version 2 Array
Samples (4)
GSM1177025 loricrin knockout
GSM1177026 wildtype
GSM1177027 loricrin knockout replicate
Relations
BioProject PRJNA209943

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE41742_RAW.tar 11.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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