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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Oct 27, 2012 |
| Title |
Distinctive microRNA signature of medulloblastomas associated with the WNT signaling pathway |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Medulloblastoma is a malignant brain tumor that occurs predominantly in children. Current risk stratification based on the clinical parameters is inadequate for accurate prognostication. In order to get a better understanding of medulloblastoma biology, miRNA profiling of medulloblastomas was carried out in parallel with the expression profiling of protein- coding genes.
miRNA profiling of medulloblastomas was carried out using Taqman Low Density Density Aarray v 1.0 having 365 human microRNAs. In parallel, genome--wide expression profiling of protein coding genes was carried out using Affymetrix gene 1.0 ST arrays. Both the profiling studies identified four molecular subtypes of medulloblastomas. Expression levels of select protein-coding genes and miRNAs could classify an independent set of medulloblastomas. Twelve of 31 medulloblastomas were found to overexpress genes belonging to the canonical WNT signaling pathway and carry a mutation in CTNNB1 gene. A number of miRNAs like miR-193a, miR-224/miR-452 cluster, miR-182/miR-183/miR-96 cluster, and miR-148a having potential tumor/metastasis suppressive activity were found to be overexpressed in the WNT signaling associated medulloblastomas. Exogenous expression of miR-193a and miR-224, two miRNAs that have the highest WNT pathway specific upregulation, was found to inhibit proliferation, increase radiation sensitivity and reduce anchorage-independent growth of medulloblastoma cells. Expression level of tumor/metastasis suppressive miRNAs in the WNT signaling associated medulloblastomas is likely to determine their response to treatment, and thus, these miRNAs would be important biomarkers for risk stratification within the WNT signaling associated medulloblastomas.
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| Overall design |
A total of 19 human sporadic medulloblastomas were profiled using the Affymetrix Gene 1.0 ST array
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| Contributor(s) |
Gokhale A, Kunder R, Goel A, Sarin R, Moiyadi A, Shenoy A, Mamidipally C, Noronha S, Kannan S, Shirsat NV |
| Citation(s) |
21358093 |
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| Submission date |
Oct 26, 2012 |
| Last update date |
Jan 08, 2019 |
| Contact name |
Neelam V Shirsat |
| E-mail(s) |
nshirsat@actrec.gov.in
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| Organization name |
Advanced Centre for Treatment, Research and Education in Cancer. Tata Memorial Centre
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| Department |
Shirsat Lab
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| Street address |
Kharghar, Sector 21
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| City |
Navi Mumbai |
| State/province |
Maharashtra |
| ZIP/Postal code |
410210 |
| Country |
India |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (19)
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| GSM1025528 |
human sporadic medulloblastoma tumor_HMED1 |
| GSM1025529 |
human sporadic medulloblastoma tumor_HMED2 |
| GSM1025530 |
human sporadic medulloblastoma tumor_HMED3 |
| GSM1025531 |
human sporadic medulloblastoma tumor_HMED5 |
| GSM1025532 |
human sporadic medulloblastoma tumor_HMED6 |
| GSM1025533 |
human sporadic medulloblastoma tumor_HMED7 |
| GSM1025534 |
human sporadic medulloblastoma tumor_HMED8 |
| GSM1025535 |
human sporadic medulloblastoma tumor_HMED9 |
| GSM1025536 |
human sporadic medulloblastoma tumor_HMED10 |
| GSM1025537 |
human sporadic medulloblastoma tumor_HMED11 |
| GSM1025538 |
human sporadic medulloblastoma tumor_HMED12 |
| GSM1025539 |
human sporadic medulloblastoma tumor_HMED13 |
| GSM1025540 |
human sporadic medulloblastoma tumor_HMED14 |
| GSM1025541 |
human sporadic medulloblastoma tumor_HMED15 |
| GSM1025542 |
human sporadic medulloblastoma tumor_HMED16 |
| GSM1025543 |
human sporadic medulloblastoma tumor_HMED17 |
| GSM1025544 |
human sporadic medulloblastoma tumor_HMED18 |
| GSM1025545 |
human sporadic medulloblastoma tumor_HMED20 |
| GSM1025546 |
human sporadic medulloblastoma tumor_HMED22 |
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| Relations |
| BioProject |
PRJNA178308 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE41842_RAW.tar |
80.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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