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| Status |
Public on Jul 15, 2013 |
| Title |
Proteomic and transcriptomic responses to thiamethoxam in the Sweetpotato whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae) |
| Organism |
Bemisia tabaci |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We investigated the transcriptional response to thiamethoxam in the Bemisia tabaci using Illumina sequencing technology. A total of 1,338 genes were differently expressed in the thiamethoxam-resistant whiteflies.
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| Overall design |
All the B biotype whiteflies were maintained on cabbage. The susceptible strain (TH-S) was cultured without exposure to any chemical insecticides. Before the sample collected, almost 10,000 thiamethoxam-resistant adult whiteflies were treated with 2,000 mg/L thiamethoxam (~LC80) to eliminate the heterozygous individuals. Then the survivors were collected after 48 hours and designated as the TH-2000 sample. Then approximately 1,500 adult TH-S and TH-2000 were collected, respectively, The RNA was extracted and sequenced using Illumina HiSeq 2000.
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| Contributor(s) |
Yang N, Xie W, Yang X, Zhou X, Zhang Y |
| Citation(s) |
23671574 |
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| Submission date |
Nov 07, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Nina Yang |
| E-mail(s) |
nina809@163.com
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| Organization name |
Chinese Academy of Agricultural Sciences
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| Street address |
12 Zhongguancun Nandajie, Haidian District
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| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100081 |
| Country |
China |
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| Platforms (1) |
| GPL16220 |
Illumina HiSeq 2000 (Bemisia tabaci) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA179139 |
| SRA |
SRP017106 |
