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| Status |
Public on Apr 25, 2013 |
| Title |
Cohesin and Polycomb proteins functionally interact to control transcription at silenced, restrained, and active genes [expression array data] |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
|
| Summary |
Cohesin is crucial for proper chromosome segregation, but also regulates gene transcription and organism development by poorly understood mechanisms. We find that in Drosophila, cohesin functionally interacts with Polycomb group (PcG) silencing proteins at both silenced and active genes. Cohesin unexpectedly facilitates binding of Polycomb Repressive Complex 1 (PRC1) to many active genes. In contrast, cohesin and PRC1 binding are mutually antagonistic at silenced genes. PRC1 depletion decreases phosphorylated RNA polymerase and mRNA at many active genes, but increases them at silenced genes. Cohesin also facilitates long-range interactions between Polycomb Response Elements in the invected-engrailed gene complex where it represses transcription. These multiple distinct cohesin-PcG interactions reveal a previously unrecognized role for PRC1 in facilitating productive gene transcription, and provide new insights into how cohesin and PRC1 control development.
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| Overall design |
We extracted RNA from control and Ph RNAi-treated BG3 cells and measured changes in gene expression following Ph dose depletion by hybridization to Affymetrix arrays. We also extracted RNA from wild-type wing imaginal disc and measured control wing disc expression levels by hybridization to Affymetrix arrays.
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| Contributor(s) |
Schaaf CA, Dorsett D |
| Citation(s) |
23818863 |
| Submission date |
Nov 07, 2012 |
| Last update date |
May 04, 2018 |
| Contact name |
Cheri A Schaaf |
| E-mail(s) |
cheri.schaaf@gmail.com
|
| Phone |
314-977-9224
|
| Organization name |
Saint Louis University School of Medicine
|
| Department |
Biochemistry & Molecular Biology
|
| Lab |
Dorsett
|
| Street address |
1100 South Grand Ave
|
| City |
St Louis |
| State/province |
MO |
| ZIP/Postal code |
63104 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
|
| Samples (9)
|
| GSM1032586 |
Mock RNAi-treated BG3 cells, biological rep1 |
| GSM1032587 |
Mock RNAi-treated BG3 cells, biological rep2 |
| GSM1032588 |
Mock RNAi-treated BG3 cells, biological rep3 |
| GSM1032589 |
Ph RNAi-treated BG3 cells, biological rep1 |
| GSM1032590 |
Ph RNAi-treated BG3 cells, biological rep2 |
| GSM1032591 |
Ph RNAi-treated BG3 cells, biological rep3 |
| GSM1032592 |
Late third instar yw wing imaginal disc, biological rep1 |
| GSM1032593 |
Late third instar yw wing imaginal disc, biological rep2 |
| GSM1032594 |
Late third instar yw wing imaginal disc, biological rep3 |
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| This SubSeries is part of SuperSeries: |
| GSE42106 |
Cohesin and Polycomb proteins functionally interact to control transcription at silenced, restrained, and active genes |
|
| Relations |
| BioProject |
PRJNA179167 |
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