|
| Status |
Public on Dec 04, 2012 |
| Title |
Rodent trophoblast epigenome |
| Organisms |
Mus musculus; Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We characterized the trophoblast stem cell epigenome and gene expression profiles in rat and mouse.
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| |
|
| Overall design |
We profiled 5 histone modifications (+ chromatin input) using ChIP-Seq, and digital expression profiles (3' RNA-Seq) for trophoblast stem cells derived from rat and mouse. Furthermore, for mouse, we profiled key trophoblast stem cell factors Elf5, Cdx2, and Eomes. We found that enhancer regions (defined as distal regions of H3K27ac/H3K4me1 enrichment) were enriched for species-specific endogenous retroviral elements.
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| |
|
| Contributor(s) |
Chuong EB, Baker JC |
| Citation(s) |
23396136 |
| |
| Submission date |
Nov 09, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Edward Chuong |
| E-mail(s) |
edward.chuong@colorado.edu
|
| Organization name |
University of Colorado Boulder
|
| Department |
BioFrontiers
|
| Lab |
Chuong
|
| Street address |
596 UCB
|
| City |
Boulder |
| ZIP/Postal code |
80304 |
| Country |
USA |
| |
|
| Platforms (4)
|
| GPL10669 |
Illumina Genome Analyzer IIx (Rattus norvegicus) |
| GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL14844 |
Illumina HiSeq 2000 (Rattus norvegicus) |
|
| Samples (26)
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|
| Relations |
| BioProject |
PRJNA179294 |
| SRA |
SRP017140 |
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