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| Status |
Public on Mar 01, 2013 |
| Title |
Expression data from injured murine kidney |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Renal recovery following injury relies on cellular regeneration. In the mouse kidney following injury, injured epithelial cells undergoes de-differentiate, proliferate and re-differentiate into functional cells, following a a tightly controlled genetic programme where specific sets of genes are up-regulated.
We used microarrays to detail the global programme of gene expression underlying cellular regeneration following injury with or without a HDAC inhibitor, m4PTB, treatment and identified distinct classes of up-regulated genes during this process.
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| Overall design |
Male BALB/c mice underwent 26 minute unilateral ischemia-reperfusion (IR) with contralateral nephrectomy treated with vehicle or 100 mg/kg m4PTB 24 h after inducing renal injury, and kidneys harvested for RNA extraction 12 h later. We sought to obtain kidneys within a similar degree of injury in order to carefully evaluate the effect of m4PTB on expression profiles. To that end, we selected kidneys according to three different criteria of injury: (1) level of serum creatinine at Day1, (2) the level of blood urea nitrogen (BUN) (3) Kim1 expression by qPCR at Day1.5 using haevested kidneys
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| Contributor(s) |
Chiba T, Skrypnik N, de Caestecker MP |
| Citation(s) |
23620402 |
| |
| Submission date |
Nov 13, 2012 |
| Last update date |
Mar 04, 2019 |
| Contact name |
James West |
| E-mail(s) |
j.west@vanderbilt.edu
|
| Organization name |
Vanderbilt University
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| Department |
Medicine
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| Lab |
Pulmonary
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| Street address |
4200 E 9TH Ave, Box B133
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37201 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA179434 |
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