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| Status |
Public on Nov 15, 2012 |
| Title |
Depth Distribution of Ammonia Oxidation Rates and Ammonia-Oxidizer Community Composition in the Sargasso Sea |
| Platform organism |
synthetic construct |
| Sample organisms |
synthetic construct; uncultured ammonia-oxidizing archaeon |
| Experiment type |
Other
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| Summary |
Ammonia oxidizer community structure were examined in a depth profile from 20 to 2000 m at the Bermuda Atlantic Time-series Study using a functional gene microarray to look at amoA diversity
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| Overall design |
Two color array (cy3 and cy5): the universal standard 20 bp oligo (fluoresced with cy5) is printed to the slide with a 70-mer. Environmental DNA sequences (fluoresced with Cy3) within 15% of the 70-mer will bind to it. Signal is the cy3/cy5. Two replicate arrays were run on duplicate targets.
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| Contributor(s) |
Newell S, Ward B, Fawcett S |
| Citation missing |
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| |
| Submission date |
Nov 14, 2012 |
| Last update date |
Nov 16, 2012 |
| Contact name |
Silvia Newell |
| E-mail(s) |
sen@bu.edu
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| Organization name |
Boston University
|
| Street address |
675 Commonwealth Ave.
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| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02215 |
| Country |
USA |
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| Platforms (1) |
| GPL16282 |
Princeton Microarray Facility AOA Archetype Microarray BC015 |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA179550 |
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