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| Status |
Public on Oct 01, 2015 |
| Title |
Clostridium difficile C630E: growth in TY with 30 mM proline vs. growth in TY alone |
| Organism |
Clostridioides difficile |
| Experiment type |
Expression profiling by array
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| Summary |
Clostridium difficile, a proteolytic Gram-positive anaerobe, has emerged as a significant nosocomial pathogen. Stickland fermentation reactions are thought to be important for growth of C. difficile. In Stickland reactions, pairs of amino acids donate and accept electrons, generating ATP and reducing power in the process. Reduction of the electron acceptors proline and glycine requires the D-proline reductase (PR) and the glycine reductase (GR) enzyme complexes, respectively. PrdR, a sigma54-dependent regulator, activates transcription of the PR-encoding genes in the presence of proline and negatively regulates the GR-encoding genes, suggesting that PrdR is a central metabolism regulator that controls preferential utilization of proline and glycine to produce energy via the Stickland reactions.
Here, transcriptional profiling of C. difficile comparing growth in TY medium supplemented with 30 mM L-proline to growth in TY medium alone is performed.
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| Overall design |
Two-condition experiment: TY with excess of proline vs. TY alone. 2 biological replicates for each condition, including a dye-swap.
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| Contributor(s) |
Bouillaut L, Dubois T, Monot M |
| Citation missing |
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| Submission date |
Nov 21, 2012 |
| Last update date |
Oct 01, 2015 |
| Contact name |
Monot Marc |
| E-mail(s) |
mmonot@pasteur.fr
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| Phone |
+33145688390
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| Organization name |
Institut Pasteur
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| Department |
Genomes and Genetics
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| Lab |
Biomics
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| Street address |
25, rue du docteur roux
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| City |
Paris |
| ZIP/Postal code |
75015 |
| Country |
France |
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| Platforms (1) |
| GPL10556 |
Agilent-020954 Clostridium difficile Expression 15.7k array |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE42472 |
Comparison of the expression profiles of the 630E JIR8094 strain and a prdR mutant in the presence of proline in TY |
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| Relations |
| BioProject |
PRJNA183177 |
