 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Nov 29, 2012 |
| Title |
An Organ Culture System to Model Early Degenerative Changes of the Intervertebral Disc: II Profiling Global Gene Expression Changes |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
|
| Summary |
To mimic a degenerative insult, rat intervertebral discs were cultured in the presence of TNF-alpha, IL-1beta and serum limiting conditions.
Gene expression analysis was performed using a microarray to identify differential gene expression between experimental and control groups.
|
| |
|
| Overall design |
The discs were collected and the annulus fibrosus (AF) was separated from the nucleus pulposus (NP). Total RNA from NP was used for RNA extraction and hybridization on Affymetrix microarrays: Control group - Ctr-1, Ctr-2, Ctr-3, Ctr-4 and Experimental group-Exp-1, Exp-2, Exp-3, Exp-4.
|
| |
|
| Contributor(s) |
Markova DZ, Kepler CK, Addya S, Murray HB, Vaccaro AR, Shapiro IM, Anderson DG, Albert TJ, Risbud MV |
| Citation(s) |
28940640, 24171898 |
| |
| Submission date |
Nov 29, 2012 |
| Last update date |
Feb 03, 2020 |
| Contact name |
Dessislava Z Markova |
| E-mail(s) |
Dessislava.Markova@Jefferson.edu
|
| Phone |
2159557655
|
| Fax |
2159559773
|
| Organization name |
Thomas Jefferson University
|
| Department |
Orthopaedic Surgery
|
| Lab |
508
|
| Street address |
1015 Walnut
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19107 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6247 |
[RaGene-1_0-st] Affymetrix Rat Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA182353 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE42611_RAW.tar |
28.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...