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| Status |
Public on Jul 01, 2014 |
| Title |
Identification and expression profiling of microRNAs in brain, liver and gonads of marine medaka (Oryzias melastigma) by Illumina small RNA Sequencing |
| Organism |
Oryzias melastigma |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
We performed miRNA transcriptome analysis of mature medaka tissues using high-throughput next generation sequencing technology. The small RNA libraries were contructed from brain, liver and gonads in mature male and female medaka and a total of more than 128 million sequencing reads were generated from the six small RNA libraries. By comparison to known miRNA database, a total of 223 known miRNA types were identified with more than 50% expressed in brain. The expression profiling showed that almost half of the miRNAs are tissue-specific, with only 55 miRNA types from 34 families common to all tissues. A small number of miRNAs are also gender-specific. The experimental validation was performed for five represented miRNA candidates and four of them are confirmed.
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| Overall design |
Investigation of miRNA expression profiles from three tissues (brain, liver and gonads) of mature male and female medaka.
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| Contributor(s) |
Lau KY, Bao JY |
| Citation missing |
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| |
| Submission date |
Dec 03, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Yunjuan Bao |
| Organization name |
University of Notre Dame
|
| Street address |
1234 Notre Dame Avenue
|
| City |
Notre Dame |
| State/province |
Indiana |
| ZIP/Postal code |
46556 |
| Country |
USA |
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| Platforms (1) |
| GPL16350 |
Illumina Genome Analyzer IIx (Oryzias melastigma) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA182747 |
| SRA |
SRP017400 |
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