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| Status |
Public on Dec 06, 2012 |
| Title |
Chromosomal changes in primary human uveal melanomas [UCSF] |
| Organism |
Homo sapiens |
| Experiment type |
Genome variation profiling by genome tiling array
|
| Summary |
Uveal melanoma is the most common cancer of the eye arising from melanocytes within the choroid, ciliary bodies and iris. Almost half of uveal melanomas metastasize hematogenously to distant organs, most often the liver, where the disease becomes fatal. One of the first genetic alterations to be identified in primary uveal melanomas was monosomy 3, which was found to be strongly associated with metastasis. We used gene expression profiling to identify two phenotypically distinct classes of uveal melanomas: class 1 tumors with low-grade morphology and low metastatic risk; and class 2 tumors with aggressive morphology and high metastatic risk. Our initial studies suggested that gene expression profiling was a better predictor of metastasis than monosomy 3. For this study, BAC-array comparative genomic hybridization was used to assay genomic DNA isolated from fresh frozen primary uveal melanoma samples of known molecular class based on gene expression profiling. Independent samples were sent to the aCGH cores at the University of California, San Francisco and the Roswell Park Cancer Institute for hybridization, and log2 ratios were reported.
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| Overall design |
Total genomic DNA was obtained from tumor samples that were collected at the time of treatment. Samples were analyzed by the Microarray Shared Resource at the Comprehensive Cancer Center, University of California, San Francisco or by the Microarray and Genomics Facility of the Roswell Park Cancer Institute.
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| |
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| Contributor(s) |
Onken MD |
| Citation(s) |
17332290 |
| |
| Submission date |
Dec 05, 2012 |
| Last update date |
Jan 03, 2013 |
| Contact name |
Michael D Onken |
| Organization name |
Washington University School of Medicine
|
| Department |
Biochemistry & Molecular Biophysics
|
| Lab |
Box 8231
|
| Street address |
660 S. Euclid Ave
|
| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
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| Platforms (1) |
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| Samples (20)
|
| GSM1049003 |
Uveal melanoma from enucleation 10 [UCSF] |
| GSM1049004 |
Uveal melanoma from enucleation 13 [UCSF] |
| GSM1049005 |
Uveal melanoma from enucleation 16 [UCSF] |
| GSM1049006 |
Uveal melanoma from enucleation 18 [UCSF] |
| GSM1049007 |
Uveal melanoma from enucleation 28 [UCSF] |
| GSM1049008 |
Uveal melanoma from enucleation 32 [UCSF] |
| GSM1049009 |
Uveal melanoma from enucleation 33 [UCSF] |
| GSM1049010 |
Uveal melanoma from enucleation 35 [UCSF] |
| GSM1049011 |
Uveal melanoma from enucleation 37 [UCSF] |
| GSM1049012 |
Uveal melanoma from enucleation 41 [UCSF] |
| GSM1049013 |
Uveal melanoma from enucleation 46 [UCSF] |
| GSM1049014 |
Uveal melanoma from enucleation 48 [UCSF] |
| GSM1049015 |
Uveal melanoma from enucleation 49 [UCSF] |
| GSM1049016 |
Uveal melanoma from enucleation 51 [UCSF] |
| GSM1049017 |
Uveal melanoma from enucleation 54 [UCSF] |
| GSM1049018 |
Uveal melanoma from enucleation 55 [UCSF] |
| GSM1049019 |
Uveal melanoma from enucleation 56 [UCSF] |
| GSM1049020 |
Uveal melanoma from enucleation 60 [UCSF] |
| GSM1049021 |
Uveal melanoma from enucleation 61 [UCSF] |
| GSM1049022 |
Uveal melanoma from enucleation 63 [UCSF] |
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| This SubSeries is part of SuperSeries: |
| GSE42740 |
Chromosomal changes in primary human uveal melanomas |
|
| Relations |
| BioProject |
PRJNA182984 |
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