|
| Status |
Public on Dec 11, 2012 |
| Title |
Expression changes in MEL cells upon differentiation and Ldb1 knockdown |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Carbonic anhydrase 1 (Car1), an early specific marker of the erythroid differentiation, has been used to distinguish fetal and adult erythroid cells since its production closely follows the γ- to β-globin transition, but the molecular mechanism underlying transcriptional regulation of Car1 is unclear. Here, we show that Car1 mRNA decreases significantly when erythroid differentiation is induced in MEL cells. The Ldb1 protein complex including GATA1/SCL/LMO2 binds to the Car1 promoter in uninduced cells and reduced enrichment of the complex during differentiation correlates with loss of Car1 expression. Knockdown of Ldb1 results in a reduction of Ser2 phosphorylated RNA Pol II and Cdk9 at the Car1 promoter region, suggesting that Ldb1 is required for recruitment of Pol II as well as the transcription regulator P-TEFb to enhance elongation of Car1 transcripts. Taken together, these data show that Ldb1 forms a regulatory complex to maintain Car1 expression in erythroid cells.
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| |
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| Overall design |
Expression analysis of induced and uninduced MEL cells after control or Ldb1 shRNA knockdown.
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| |
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| Contributor(s) |
Dale RK, Dean A |
| Citation(s) |
22609543 |
| |
| Submission date |
Dec 11, 2012 |
| Last update date |
Dec 13, 2012 |
| Contact name |
Ryan Dale |
| E-mail(s) |
dalerr@nih.gov
|
| Organization name |
National Institutes of Health
|
| Department |
National Institute of Child Health and Human Development
|
| Lab |
Bioinformatics and Scientific Programming Core
|
| Street address |
Rm 10D39, 10 Center Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16368 |
[Mouse4302_Mm_ENTREZG] Affymetrix GeneChip Mouse Genome 430 2.0 Array [Brainarray Version 16] |
|
| Samples (10)
|
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| Relations |
| BioProject |
PRJNA183613 |
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