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| Status |
Public on Apr 03, 2013 |
| Title |
The effect of nicotinamide on dysregulated genes associated with frataxin deficiency in FRDA. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To investigate the efficacy of nicotinamide treatment using our ex-vivo primary lymphocyte model, we performed high-throughput RNA sequencing on libraries generated from untreated and nicotinamide treated samples.
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| |
|
| Overall design |
PBMC isolated from FRDA affected individuals were cultured to prepare the primary lymphocyte cell lines. The primary cultured cells were either treated with 10mM nicotinamide or without the addition of drug during the 3-days treatment. RNA was extracted after the treatment and then RNA-seq libraries were generated by standard protocols.
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| |
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| Contributor(s) |
Chan PK, Khadayate S |
| Citation(s) |
23474817 |
| |
| Submission date |
Dec 17, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Sanjay Padmakar Khadayate |
| Organization name |
MRC London Institute of Medical Sciences
|
| Street address |
Du Cane Road
|
| City |
London |
| ZIP/Postal code |
W12 0NN |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (4)
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| Relations |
| BioProject |
PRJNA183943 |
| SRA |
SRP017580 |
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