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Status |
Public on Apr 03, 2013 |
Title |
Expression data from a human MLL-AF6 positive AML cell line |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
The MLL gene on chromosome 11 fuses to the AF6 gene on chromosome 6 in a balanced chromosomal translocation that is characetristic of certain adult and pediatric human leukemias. We established a murine leukemia model of MLL-AF6 using the retroviral MLL-AF6 contruct in a bone marrow transplantation system. The ML2 human MLL-AF6 positive leukemia cell line was used for gene expression profiling to assess the transctiptional profile in MLL-AF6 leukemias.
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Overall design |
RNA was extracted from the ML2 cell line cultured under standard conditionswith TRIZOL (Invitrogen), purified using RNAeasy(Qiagen) and hybridized onto Affymetrix arrays.
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Contributor(s) |
Sinha A, Deshpande A, Armstrong S |
Citation(s) |
23361907 |
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Submission date |
Dec 20, 2012 |
Last update date |
Mar 25, 2019 |
Contact name |
Amit Sinha |
E-mail(s) |
amit.sinha@childrens.harvard.edu
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Phone |
617-582-7579
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Organization name |
Dana-Farber Cancer Institute
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Department |
Pediatric Oncology
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Lab |
Armstrong Lab
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Street address |
44 Binney St
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City |
Boston |
State/province |
MA |
ZIP/Postal code |
02135 |
Country |
USA |
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Platforms (1) |
GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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Samples (1) |
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This SubSeries is part of SuperSeries: |
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Relations |
BioProject |
PRJNA184355 |