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Status |
Public on Jun 15, 2006 |
Title |
Effect of posttranslational modifications of SATB1 on its ability to regulate global gene expression in HeLa Cells |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Gene profiling studies using RNA from HeLa cells overexpressing point mutants of SATB1 defective in phosphorylation or acetylation unequivocally demonstrated the importance of these modifications towards the ability of SATB1 to act as a global regulator of gene expression. HeLa cells were chosen as a representative of non-T cells, and do not express SATB1 endogenously. Keywords: cDNA array
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Overall design |
Total RNA from untransfected HeLa cells was used as reference and was labeled with Cy5. Total RNA from HeLa cells stably transfected with wild type, or S185A, or K136A mutant SATB1 expressing construct was used as experimental sample and was labeled with Cy3.
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Contributor(s) |
Kumar P, Notani D, Kumar C, Galande S |
Citation missing |
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Submission date |
Mar 01, 2006 |
Last update date |
Mar 17, 2012 |
Contact name |
Sanjeev Galande |
E-mail(s) |
sanjeev@nccs.res.in
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Phone |
91-20-25690922
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Fax |
91-20-25692259
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Organization name |
National Centre for Cell Science
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Lab |
Chromatin Biology Laboratory
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Street address |
Ganeshkhind
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City |
Pune |
ZIP/Postal code |
411007 |
Country |
India |
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Platforms (1) |
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Samples (3) |
GSM98547 |
Global regulation of gene expression by SATB1 in HeLa cells from Galande Lab |
GSM98585 |
Effect of S185A mutation on global gene regulation by SATB1 in HeLa cells from Galande Lab |
GSM98586 |
Effect of K136A mutation on global gene regulation by SATB1 in HeLa cells from Galande Lab |
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This SubSeries is part of SuperSeries: |
GSE4317 |
Effect of post-translational modifications of SATB1 on global gene expression in HeLa and Jurkat T cells |
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Relations |
BioProject |
PRJNA104649 |