|
| Status |
Public on Jan 22, 2013 |
| Title |
MicroRNA regulate immunological pathways in T-cells in immune thrombocytopenia (ITP) [miRNA] |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
MicroRNA are small non-coding RNA molecules that regulate gene expression. To investigate the role of microRNA in ITP, we performed genome-wide expression analyses of mRNA and microRNA in T-cells from ITP patients and controls. We identified 1,915 regulated genes and 22 regulated microRNA that differed between ITP patients and controls. Seventeen of the 22 regulated microRNA were linked to changes in target gene expression; 57 of these target genes were associated with the immune system, e.g. T-cell activation and regulation of immunoglobulin production. CXCL13 and IL-21 were two microRNA target genes significantly increased in ITP. We could demonstrate increased plasma levels of CXCL13 and others have reported increased plasma levels of IL-21 in ITP. Thus, regulated microRNA were significantly associated with both gene and protein expression of molecules in immunological pathways. We suggest that microRNA may be important regulatory molecules involved in the loss of tolerance in ITP.
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| |
|
| Overall design |
Microarray expression analysis of mRNA and miRNA in peripheral blood T-cell of control and ITP patients.
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| |
|
| Contributor(s) |
Jernås M, Nookaew I, Wadenvik H, Olsson B |
| Citation(s) |
23303824 |
| |
| Submission date |
Dec 28, 2012 |
| Last update date |
May 02, 2017 |
| Contact name |
Intawat Nookaew |
| E-mail(s) |
inookaew@uams.edu
|
| Organization name |
University of Arkansas for Medical Sciences
|
| Department |
Department of Biomedical Informatics
|
| Street address |
4301 W Markham St
|
| City |
Little Rock |
| ZIP/Postal code |
72205 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL14613 |
[miRNA-2] Affymetrix Multispecies miRNA-2 Array |
|
| Samples (18)
|
| GSM1057962 |
peripheral blood T-cells Control1 [miRNA] |
| GSM1057963 |
peripheral blood T-cells Control2 [miRNA] |
| GSM1057964 |
peripheral blood T-cells Control3 [miRNA] |
| GSM1057965 |
peripheral blood T-cells Control4 [miRNA] |
| GSM1057966 |
peripheral blood T-cells Control6 [miRNA] |
| GSM1057967 |
peripheral blood T-cells Control7 [miRNA] |
| GSM1057968 |
peripheral blood T-cells Control8 [miRNA] |
| GSM1057969 |
peripheral blood T-cells Control10 [miRNA] |
| GSM1057970 |
peripheral blood T-cells Control11 [miRNA] |
| GSM1057971 |
peripheral blood T-cells Patient1 [miRNA] |
| GSM1057972 |
peripheral blood T-cells Patient2 [miRNA] |
| GSM1057973 |
peripheral blood T-cells Patient3 [miRNA] |
| GSM1057974 |
peripheral blood T-cells Patient4 [miRNA] |
| GSM1057975 |
peripheral blood T-cells Patient5 [miRNA] |
| GSM1057976 |
peripheral blood T-cells Patient6 [miRNA] |
| GSM1057977 |
peripheral blood T-cells Patient7 [miRNA] |
| GSM1057978 |
peripheral blood T-cells Patient8 [miRNA] |
| GSM1057979 |
peripheral blood T-cells Patient9 [miRNA] |
|
| This SubSeries is part of SuperSeries: |
| GSE43179 |
MicroRNA regulate immunological pathways in T-cells in immune thrombocytopenia (ITP) |
|
| Relations |
| BioProject |
PRJNA184852 |
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