|
| Status |
Public on Jan 31, 2013 |
| Title |
Antitumor activity of a pyrrole-imidazole polyamide |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We report that a DNA minor groove binding hairpin pyrrole-imidazole (Py-Im) polyamide interferes with RNA polymerase II (RNAP2) activity in cell culture. Genome-wide mapping of RNAP2 binding shows reduction of occupancy preferentially at transcription start sites (TSS), while occupancy at enhancer sites are is unchanged.
|
| |
|
| Overall design |
Genome-wide mapping of RNA polymerase II in LNCaP cells treated with DHT and DHT with Py-Im polyamide.
|
| |
|
| Contributor(s) |
Yang F |
| Citation(s) |
23319609 |
| |
| Submission date |
Jan 02, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Fei Yang |
| Organization name |
California Institute of Technology
|
| Street address |
1200 E. California Blvd.
|
| City |
Pasadena |
| State/province |
CA |
| ZIP/Postal code |
91125 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA185145 |
| SRA |
SRP017754 |
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