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| Status |
Public on Jul 28, 2013 |
| Title |
Comparative expression profiling of miRNA during anther development in genetic male sterile and wild type cotton [degradome] |
| Organism |
Gossypium hirsutum |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
In this study, in order to identify miRNA targets, a degradome library derived from anthers of the WT and GMS (Genetic Male Sterility) mutant representing three stages of development was constructed and sequenced, resulting in the generation of 24.6 million raw reads. After removal of low quality sequences and adapter sequences, 24.4 million clean reads were obtained and 98% were 20 or 21 nt in length as expected in that normally length distribution peak of degradome fragment is between 20 and 21 nt [Addo-Quaye C, Eshoo TW, Bartel DP, Axtell MJ: Endogenous siRNA and miRNA targets identified by sequencing of the Arabidopsis degradome. Curr Biol 2008, 18:758-762].
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| Overall design |
Identification of miRNA targets in the WT and GMS muant anthers.
Anthers of the WT and GMS mutant representing three stages of development [the meiosis stage (WT: Mar-F-1; mutant: Mar-S-1) and tetrad stage (WT: Mar-F-2; mutant: Mar-S-2), together with the uninucleate microspore stage (WT: Mar-F-3; mutant: Mar-S-3) from the GMS ‘Dong A’ mutant and its fertile wild type] were collected during early mornings.
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| Contributor(s) |
Wei Mm, Yu Sx |
| Citation missing |
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| Submission date |
Jan 09, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Ming ming Wei |
| E-mail(s) |
lywmm@yahoo.cn
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| Phone |
86-372-2525365
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| Organization name |
Cotton Research Institute, Chinese Academy of Agriculture Sciences (CAAS)
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| Street address |
Huang he road 38
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| City |
Anyang |
| ZIP/Postal code |
455000 |
| Country |
China |
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| Platforms (1) |
| GPL16485 |
Illumina HiSeq 2000 (Gossypium hirsutum) |
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| Samples (1) |
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| This SubSeries is part of SuperSeries: |
| GSE43532 |
The role of microRNA-regulating energy metabolism and pollen wall development in male sterility in cotton |
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| Relations |
| BioProject |
PRJNA185810 |
| SRA |
SRP017861 |
