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| Status |
Public on Sep 05, 2013 |
| Title |
RIP-Chip of endogenous Staufen and GFP-tagged Staufen from 0-3 hour Drosophila embryos |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
|
| Summary |
Staufen-associated mRNAs were identified in early Drosophila embryos by RNA-co-immunoprecipitation followed by microarray analysis (RIP-Chip), using two complementary approaches: RIP-Chip from wild-type embryos using a synthetic anti-Staufen antibody, and RIP-Chip from transgenic GFP-Staufen-expressing embryos using an anti-GFP antibody. Genome-wide transcript expression in whole embryos was also assessed for the wild-type and GFP-Staufen lines.
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| Overall design |
There are 18 samples in total. These include 3 replicates each of 1) gene expression profiling from total mRNA isolated from wild-type 0-3 hour embryos, 2) synthetic anti-Staufen antibody RNA co-immunoprecipitations of endogenous Staufen from wild-type 0-3 hour embryos, 3) control synthetic antibody RNA co-immunoprecipitations from wild-type 0-3 hour embryos, 4) gene expression profiling from total mRNA isolated from transgenic GFP-Staufen expressing 0-3 hour embryos, 5) anti-GFP RNA co-immunoprecipitations of GFP-Staufen from transgenic GFP-Staufen expressing 0-3 hour embryos, 6) anti-FLAG control RNA co-immunoprecipitations from transgenic GFP-Staufen expressing 0-3 hour embryos.
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| Contributor(s) |
Laver JD, Ancevicius K, Li X, Morris Q, Westwood JT, Smibert CA, Lipshitz HD |
| Citation(s) |
23945942 |
| |
| Submission date |
Jan 10, 2013 |
| Last update date |
Sep 05, 2013 |
| Contact name |
Howard D Lipshitz |
| E-mail(s) |
howard.lipshitz@utoronto.ca
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| Organization name |
University of Toronto
|
| Department |
Molecular Genetics
|
| Street address |
1 King's College Circle
|
| City |
Toronto |
| State/province |
ON |
| ZIP/Postal code |
M5S1A8 |
| Country |
Canada |
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| Platforms (1) |
| GPL10539 |
CDMC Nimblegen Drosophila 17K v1 |
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| Samples (18)
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| GSM1062252 |
Drosophila_melanogaster_w1118_0-3h_embryo_total_RNA_rep1 |
| GSM1062253 |
Drosophila_melanogaster_w1118_0-3h_embryo_total_RNA_rep2 |
| GSM1062254 |
Drosophila_melanogaster_w1118_0-3h_embryo_total_RNA_rep3 |
| GSM1062255 |
Drosophila_melanogaster_synthetic_anti-Staufen_RIP_RNA_rep1 |
| GSM1062256 |
Drosophila_melanogaster_synthetic_anti-Staufen_RIP_RNA_rep2 |
| GSM1062257 |
Drosophila_melanogaster_synthetic_anti-Staufen_RIP_RNA_rep3 |
| GSM1062258 |
Drosophila_melanogaster_synthetic_control_RIP_RNA_rep1 |
| GSM1062259 |
Drosophila_melanogaster_synthetic_control_RIP_RNA_rep2 |
| GSM1062260 |
Drosophila_melanogaster_synthetic_control_RIP_RNA_rep3 |
| GSM1062261 |
Drosophila_melanogaster_GFP-Staufen_0-3h_embryo_total_RNA_rep1 |
| GSM1062262 |
Drosophila_melanogaster_GFP-Staufen_0-3h_embryo_total_RNA_rep2 |
| GSM1062263 |
Drosophila_melanogaster_GFP-Staufen_0-3h_embryo_total_RNA_rep3 |
| GSM1062264 |
Drosophila_melanogaster_anti-GFP_RIP_RNA_rep1 |
| GSM1062265 |
Drosophila_melanogaster_anti-GFP_RIP_RNA_rep2 |
| GSM1062266 |
Drosophila_melanogaster_anti-GFP_RIP_RNA_rep3 |
| GSM1062267 |
Drosophila_melanogaster_anti-FLAG_RIP_RNA_rep1 |
| GSM1062268 |
Drosophila_melanogaster_anti-FLAG_RIP_RNA_rep2 |
| GSM1062269 |
Drosophila_melanogaster_anti-FLAG_RIP_RNA_rep3 |
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| Relations |
| BioProject |
PRJNA186029 |
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