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| Status |
Public on Jan 01, 2015 |
| Title |
Quantitative proteomics cell bilayer (qPCB) method for studying cell-cell communication |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Cellular communication is a fundamental process in biology. We developed an in vitro procedure for quantitatively analyzing proteome-wide changes triggered by interaction of different cell types. Using an adipocyte-macrophage bilayer co-culture model we detected thousands of proteins and deciphered regulatory pathways involved in low-grade inflammation leading to insulin resistance. The method can be applied for multiple cell-cell combinations to gain understanding in cellular interaction.
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| Overall design |
2 cell lines (3T3-L1 adipocytes [A] and RAW 264.7 macrophages [R]), cultivated both as monolayer (condition 1 [O]) and together in a bilayer experiment (condition 2 [B]); 2-3 biological replicates per treatment (10 samples in total)
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| Contributor(s) |
Christopher W, Anja F, Sascha S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jan 10, 2013 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Christopher Weidner |
| E-mail(s) |
weidner@molgen.mpg.de
|
| Organization name |
Max Planck-Institute for Molecular Genetics
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| Department |
Otto Warburg Laboratory
|
| Lab |
Sauer
|
| Street address |
Ihnestraße 63
|
| City |
Berlin |
| ZIP/Postal code |
14195 |
| Country |
Germany |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA186076 |
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