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| Status |
Public on Aug 30, 2013 |
| Title |
Co-ordinate inhibition of autism candidate genes by topoisomerase inhibitors [Seq] |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Topoisomerases are necessary for the expression of neurodevelopmental genes, and are mutated in some patients with autism spectrum disorder (ASD). We have studied the effects of inhibitors of Topoisomerase 1 (Top1) and Topoisomerase 2 (Top2) enzymes on mouse cortical neurons. We find that topoisomerases selectively inhibit long genes (>100kb), with little effect on all other gene expression. Using ChIPseq against RNA Polymerase II (Pol2) we show that the Top1 inhibitor topotecan blocks transcriptional elongation of long genes specifically. Many of the genes inhibited by topotecan are candidate ASD genes, leading us to propose that topoisomerase inhibition might contribute to ASD pathology.
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| Overall design |
[Mouse] 5 biological replicates of transcriptome sequencing (RNAseq) from topotecan treated neurons and vehicle treated controls; Pol2 ChIPseq of topotecan and vehicle treated neurons
[Human] Transcriptome sequencing (RNAseq) from topotecan treated neurons and vehicle treated control.
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| |
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| Contributor(s) |
King IF, Yandava C |
| Citation(s) |
23995680 |
| |
| Submission date |
Jan 16, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Chandri Yandava |
| E-mail(s) |
yandava@email.unc.edu
|
| Organization name |
University of North Carolina at Chapel Hill
|
| Department |
Carolina Institute for Developmental Disabilities
|
| Street address |
101 Renee Lynne Court
|
| City |
Carrboro |
| State/province |
NC |
| ZIP/Postal code |
27510 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (14)
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| This SubSeries is part of SuperSeries: |
| GSE43900 |
Co-ordinate inhibition of autism candidate genes by topoisomerase inhibitors |
|
| Relations |
| BioProject |
PRJNA186671 |
| SRA |
SRP017966 |
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