|
| Status |
Public on Aug 02, 2013 |
| Title |
MicroRNA regulate immune pathways in T-cells in multiple sclerosis (MS) miRNA |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
MicroRNAs are small noncoding RNA molecules that are involved in the control of gene expression. To investigate the role of microRNA in multiple sclerosis (MS), we performed global microarray analyses of mRNA and microRNA in peripheral blood T-cells from relapsing-remitting MS patients and controls. We identified 2,452 regulated genes and 21 regulated microRNA that differed between MS patients and controls. By Kolmogorov-Smirnov test, 20 of 21 regulated microRNA were shown to affect the expression of their target genes, many of which are involved in the immune system. LIGHT (TNFSF14) was a microRNA target gene significantly decreased in MS. The down-regulation of mir-494 and predicted mRNA-target LIGHT was verified by real-time PCR and we could demonstrate decreased serum levels of LIGHT in MS. Thus, regulated microRNA were significantly associated with both gene and protein expression of a molecule in immunological pathways. These findings indicate that microRNA may be important regulatory molecules in T-cells in MS.
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| |
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| Overall design |
Microarray expression analysis of mRNA and miRNA in peripheral blood T-cell of control and MS patients
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| |
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| Contributor(s) |
Jernas M, Malmestrom C, Axelsson M, Nookaew I, Wadenvik H, Lycke J, Olsson B |
| Citation(s) |
23895517 |
| |
| Submission date |
Jan 17, 2013 |
| Last update date |
May 02, 2017 |
| Contact name |
Intawat Nookaew |
| E-mail(s) |
inookaew@uams.edu
|
| Organization name |
University of Arkansas for Medical Sciences
|
| Department |
Department of Biomedical Informatics
|
| Street address |
4301 W Markham St
|
| City |
Little Rock |
| ZIP/Postal code |
72205 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL14613 |
[miRNA-2] Affymetrix Multispecies miRNA-2 Array |
|
| Samples (20)
|
| GSM1065985 |
peripheral blood T-cells miRNA Patient1 |
| GSM1065986 |
peripheral blood T-cells miRNA Patient2 |
| GSM1065987 |
peripheral blood T-cells miRNA Patient3 |
| GSM1065988 |
peripheral blood T-cells miRNA Patient4 |
| GSM1065989 |
peripheral blood T-cells miRNA Patient5 |
| GSM1065990 |
peripheral blood T-cells miRNA Patient6 |
| GSM1065991 |
peripheral blood T-cells miRNA Patient7 |
| GSM1065992 |
peripheral blood T-cells miRNA Patient8 |
| GSM1065993 |
peripheral blood T-cells miRNA Patient9 |
| GSM1065994 |
peripheral blood T-cells miRNA Patient10 |
| GSM1065995 |
peripheral blood T-cells miRNA Patient11 |
| GSM1065996 |
peripheral blood T-cells miRNA control1 |
| GSM1065997 |
peripheral blood T-cells miRNA control2 |
| GSM1065998 |
peripheral blood T-cells miRNA control3 |
| GSM1065999 |
peripheral blood T-cells miRNA control4 |
| GSM1066000 |
peripheral blood T-cells miRNA control6 |
| GSM1066001 |
peripheral blood T-cells miRNA control7 |
| GSM1066002 |
peripheral blood T-cells miRNA control8 |
| GSM1066003 |
peripheral blood T-cells miRNA control10 |
| GSM1066004 |
peripheral blood T-cells miRNA control11 |
|
| This SubSeries is part of SuperSeries: |
| GSE43592 |
MicroRNA regulate immune pathways in T-cells in multiple sclerosis (MS) |
|
| Relations |
| BioProject |
PRJNA186784 |
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