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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 09, 2013 |
| Title |
Differentiation of human fetal multipotential neural progenitor cells to astrocytes reveals susceptibility factors for JC Virus |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Viral infections of the CNS are of increasing concern, especially among immunocompromised populations. Rodent models are often inappropriate for studies of CNS infection, as many viruses, including JC Virus (JCV) and HIV, cannot replicate in rodent cells. Consequently, human fetal brain-derived multipotential CNS progenitor cells (NPCs) that can be differentiated into neurons, oligodendrocytes, or astrocytes, have served as a model for CNS studies. NPCs can be non-productively infected by JCV, while infection of progenitor-derived astrocytes (PDAs) is robust. We profiled cellular gene expression at multiple times during differentiation of NPCs to PDAs. Several activated transcription factors show commonality between cells of the brain in which JCV replicates and lymphocytes in which JCV is likely latent. Bioinformatic analysis determined transcription factors that may influence the favorable transcriptional environment for JCV in PDAs. This study attempts to provide a framework for understanding the functional transcriptional profile necessary for productive JCV infection.
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| Overall design |
19 Human samples: 4 Human Fetal Brain NPC 0h, 4 Human Fetal Brain NPC in Serum 1h, 4 Human Fetal Brain NPC in Serum 1d, 4 Human Fetal Brain NPC in Serum 7d, 3 Human Fetal Brain NPC in Serum 30d.
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| Contributor(s) |
Ferenczy MW, Johnson KR, Marshall LJ, Monaco MC, Major EO |
| Citation(s) |
23536657 |
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| Submission date |
Jan 28, 2013 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Kory R Johnson |
| E-mail(s) |
johnsonko@ninds.nih.gov
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| Phone |
301-402-1956
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| Organization name |
NINDS/NIH
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| Department |
DIR IT & Bioinformatics
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| Lab |
Bioinformatics Section
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| Street address |
10/3B01, 9000 Rockville Pike
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (19)
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| GSM1071340 |
Human Fetal Brain NPC 0h Replicate 4 |
| GSM1071341 |
Human Fetal Brain NPC in Serum 1h Replicate 1 |
| GSM1071342 |
Human Fetal Brain NPC in Serum 1h Replicate 2 |
| GSM1071343 |
Human Fetal Brain NPC in Serum 1h Replicate 3 |
| GSM1071344 |
Human Fetal Brain NPC in Serum 1h Replicate 4 |
| GSM1071345 |
Human Fetal Brain NPC in Serum 1d Replicate 1 |
| GSM1071346 |
Human Fetal Brain NPC in Serum 1d Replicate 2 |
| GSM1071347 |
Human Fetal Brain NPC in Serum 1d Replicate 3 |
| GSM1071348 |
Human Fetal Brain NPC in Serum 1d Replicate 4 |
| GSM1071349 |
Human Fetal Brain NPC in Serum 7d Replicate 1 |
| GSM1071350 |
Human Fetal Brain NPC in Serum 7d Replicate 2 |
| GSM1071351 |
Human Fetal Brain NPC in Serum 7d Replicate 3 |
| GSM1071352 |
Human Fetal Brain NPC in Serum 7d Replicate 4 |
| GSM1071353 |
Human Fetal Brain NPC in Serum 30d Replicate 1 |
| GSM1071354 |
Human Fetal Brain NPC in Serum 30d Replicate 2 |
| GSM1071355 |
Human Fetal Brain NPC in Serum 30d Replicate 3 |
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| Relations |
| BioProject |
PRJNA187988 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE43794_RAW.tar |
87.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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