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| Status |
Public on Aug 01, 2014 |
| Title |
Association of microRNAs with colon tumor metastases |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
We compared the expression of microRNAs in primary colon tumors with their paired liver metastases using the Nanostring human microRNA assays version 1
23 microRNAs were found to be differentially expressed between colon primary tumors and their paired metastases
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| |
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| Overall design |
Total mRNA was extracted from flash frozen primary colon tumors and paired metastases. 18 tissues from 9 donors were used for this study. 100 ng of RNA was hybridized to Nanostring Human 0microRNA assays version 1.0. Data was normalized according to manufacterer's recommendation. This included background substraction and normalizing to the geometric mean of the highest 75 microRNAs.
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| Contributor(s) |
Schetter AJ, Harris CC |
| Citation missing |
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| |
| Submission date |
Feb 06, 2013 |
| Last update date |
Aug 02, 2014 |
| Contact name |
Aaron Schetter |
| E-mail(s) |
schettera@mail.nih.gov
|
| Organization name |
National Cancer Institute
|
| Department |
Laboratory of Human Carcinogenesis
|
| Street address |
37 Convent Drive, Building 37, Room 3050
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL16231 |
Nanostring nCounter Human microRNA Expression Platform |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA188717 |
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