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Series GSE44286 Query DataSets for GSE44286
Status Public on Feb 13, 2013
Title Master Transcription Factors and Mediator Establish Super-Enhancers at Key Cell Identity Genes [ChIP-Seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The master transcription factors Oct4, Sox2 and Nanog bind enhancer elements and recruit the Mediator co-activator to activate much of the gene expression program of embryonic stem cells (ESCs). We report here that the ESC master transcription factors and Mediator form “super-enhancers” at most genes known to control the pluripotent state, including those encoding the master transcription factors themselves. These super-enhancers consist of extraordinarily large genomic domains occupied by exceptional amounts of Oct4, Sox2, Nanog, Klf4, Esrrb and Mediator. Super-enhancers stimulate considerably higher transcription than typical enhancers in vivo and in reporter vectors. Reduced levels of Oct4 or Mediator cause preferential loss of expression of super-enhancer-associated genes relative to other genes, suggesting how changes in gene expression programs might be accomplished during development. In other more differentiated cells, super-enhancers containing cell-type-specific master transcription factors are also found at genes that define cell identity. These results implicate super-enhancers in the control of mammalian cell identity and differentiation.
Overall design ChIP-Seq and controls associated with Super-Enhancers in murine cell types
Contributor(s) Newman JJ, Bilodeau S, Mullen AC, Reddy J, Whyte W, Orlando D, Abraham BJ, Hnisz D, Young RA
Citation(s) 23582322
Submission date Feb 12, 2013
Last update date May 15, 2019
Contact name Richard A Young
Phone 617-258-5219
Organization name Whitehead Institute for Biomedical Research
Lab Young Lab
Street address 9 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
Platforms (3)
GPL9185 Illumina Genome Analyzer (Mus musculus)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (9)
GSM1082340 ChIP-Seq_Oct4_V65
GSM1082341 ChIP-Seq_Sox2_V65
GSM1082342 ChIP-Seq_Nanog_V65
This SubSeries is part of SuperSeries:
GSE44288 Master Transcription Factors and Mediator Establish Super-Enhancers at Key Cell Identity Genes
BioProject PRJNA189323
SRA SRP018619

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Supplementary file Size Download File type/resource
GSE44286_RAW.tar 324.6 Mb (http)(custom) TAR (of WIG)
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Processed data provided as supplementary file
Raw data are available in SRA

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