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| Status |
Public on Dec 31, 2017 |
| Title |
Microarray global microRNA expression of donor cells, fibroblast-derived iPSC, and stromal-primed cord-blood iPSC |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Global microRNA analysis of (a) human embryonic stem cells, (b) low passage episomal stromal-primed myeloid iPSC, (c) intermediate passage fibroblast iPSC, and (d) samples of corresponding donor cells.
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| Overall design |
Human miRNA Microarray Kit Version 3 kits (Agilent Technologies, Santa Clara, CA) were used for microarray assays to examine the global microRNA expression of human ES cells, iPS cells and target cell populations. The Human miRNA Microarray contains probes for 866 human microRNAs from the Sanger database v12.0. Total RNA was harvested from indicated donor cells, hESC, or iPSC, and prepared as described in the miRNeasy Mini Kit (Qiagen) with on-column DNase I digestion. A single sample of each cell line was used for this analysis.
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| Contributor(s) |
Park TS, Huo JS, Talbot CC, Yu W, Verma K, Peters A, Reijo-Pera R, Zambidis ET |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Feb 20, 2013 |
| Last update date |
Mar 14, 2018 |
| Contact name |
Elias Thomas Zambidis |
| Organization name |
Johns Hopkins School of Medicine
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| Department |
Oncology
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| Street address |
733 N. Broadway
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| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
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| Platforms (1) |
| GPL10850 |
Agilent-021827 Human miRNA Microarray (V3) (miRBase release 12.0 miRNA ID version) |
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| Samples (21)
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| GSM1084887 |
Cord Blood CD34+ progenitors Sample 1 |
| GSM1084888 |
mobilized peripheral blood CD34+ progenitors |
| GSM1084889 |
Bone Marrow CD34+ progenitors |
| GSM1084890 |
P1-FL-Bulk Reprogrammed Cells |
| GSM1084891 |
P1-CB-Bulk Reprogrammed Cells |
| GSM1084892 |
P1-mPB-Bulk Reprogrammed Cells |
| GSM1084893 |
P1-BM-Bulk Reprogrammed Cells |
| GSM1084894 |
hESC (H9) p45 |
| GSM1084895 |
nonviral 7F stromal primed cord blood iPSC clone 6.2 p14 |
| GSM1084896 |
nonviral 7F stromal primed cord blood iPSC clone 6.11 p14 |
| GSM1084897 |
nonviral 7F stromal primed cord blood iPSC clone 6.13 p14 |
| GSM1084898 |
nonviral 7F stromal primed cord blood iPSC clone 19.11 p14 |
| GSM1084899 |
nonviral Keratinocyte iPSC clone 1 p14 |
| GSM1084900 |
nonviral Keratinocyte iPSC clone 1 p13 |
| GSM1084901 |
nonviral 7F Adult fibroblast iPSC clone 6.1 p12 |
| GSM1084902 |
nonviral 7F Adult fibroblast iPSC clone 6.6 p12 |
| GSM1084903 |
viral fibroblast iPSC line IMR90-1 |
| GSM1084904 |
viral fibroblast iPSC line IMR90-4 |
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| This SubSeries is part of SuperSeries: |
| GSE82233 |
Quality of reprogramming to induced pluripotency as a function of reprogramming method |
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| Relations |
| BioProject |
PRJNA189959 |
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