 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Sep 13, 2016 |
| Title |
Global gene expression analysis of human embryonic stem cells, adult fibroblasts, and CD34+ cord blood (CB) cells before, during, and after generation of stromal primed and non-stromal primed episomal induction of pluripotency |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Global gene expression analysis of (a) human embryonic stem cells, (b) adult fibroblasts with and without nucleofection of SOKM, (c) CD34+ cord blood cells at various time points during induction of pluripotency with SOKM, with or without co-culture with bone marrow stromal cells (BMSC), and (d) resulting stromal primed and non-stromal primed cord blood CD34+ myeloid iPSC
|
| |
|
| Overall design |
Total RNA was harvested from adult fibroblasts, adult fibroblasts three days after nucleofection with 4F (SOKM), human myeloid progenitors: Non-nucloefected CD34+ Day -3 unstimulated cord blood (CB) cells, Day 0 growth factor stimulated CB cells, Day +3 CB cells with or without nucleofection at Day zero and with or without Day zero to Day +3 co-culture with bone marrow stromal cells (BMSC), followed by FACS-purification of CD45+ myeloid progenitors, Day 23 CB cells nucleofected on day zero and co-cultured on BMSC from Day Zero to Day +3, undifferentiated H9 human embryonic stem cells, and low passage episomal stromal-primed iPSC derived from growth-factor activated CD34+ myeloid progenitors from cord blood or GCSF mobilized peripheral blood. Illumina HumanHT-12 V4.0 expression beadchips were used for all samples in this series. Three independent samples of each condition were each run on individual microarrays.
|
| |
|
| Contributor(s) |
Park TS, Huo JS, Talbot CC, Yu W, Verma K, Zimmerlin L, Peters A, Kaplan I, Hu K, Slukvin I, Zambidis ET |
| Citation(s) |
27660325, 27688775 |
| |
| Submission date |
Feb 20, 2013 |
| Last update date |
Dec 13, 2016 |
| Contact name |
Elias Thomas Zambidis |
| Organization name |
Johns Hopkins School of Medicine
|
| Department |
Oncology
|
| Street address |
733 N. Broadway
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL10904 |
Illumina HumanHT-12 V4.0 expression beadchip (gene symbol) |
|
| Samples (46)
|
| GSM1084951 |
Fibroblast Day+3 +4F Experiment 1 |
| GSM1084952 |
Fibroblast Day+3 +4F Experiment 2 |
| GSM1084953 |
Fibroblast Day+3 +4F Experiment 3 |
| GSM1084954 |
Cord Blood Day -3, Experiment 1 |
| GSM1084955 |
Cord Blood Day -3, Experiment 2 |
| GSM1084956 |
Cord Blood Day -3, Experiment 3 |
| GSM1084957 |
Cord Blood Day zero, Experiment 1 |
| GSM1084958 |
Cord Blood Day zero, Experiment 2 |
| GSM1084959 |
Cord Blood Day zero, Experiment 3 |
| GSM1084960 |
Cord Blood Day zero, Experiment 4 |
| GSM1084961 |
Cord Blood Day zero, Experiment 5 |
| GSM1084962 |
Cord Blood Day +3 no 4F, no BMSC, Experiment 1 |
| GSM1084963 |
Cord Blood Day +3 no 4F, no BMSC, Experiment 2 |
| GSM1084964 |
Cord Blood Day +3 no 4F, no BMSC, Experiment 3 |
| GSM1084965 |
Cord Blood Day +3 no 4F, +BMSC, Experiment 1 |
| GSM1084966 |
Cord Blood Day +3 no 4F, +BMSC, Experiment 2 |
| GSM1084967 |
Cord Blood Day +3 no 4F, +BMSC, Experiment 3 |
| GSM1084968 |
Cord Blood Day +3 +4F, no BMSC, Experiment 1 |
| GSM1084969 |
Cord Blood Day +3 +4F, no BMSC, Experiment 2 |
| GSM1084970 |
Cord Blood Day +3 +4F, no BMSC, Experiment 3 |
| GSM1084971 |
Cord Blood Day +3 +4F, +BMSC, Experiment 1 |
| GSM1084972 |
Cord Blood Day +3 +4F, +BMSC, Experiment 2 |
| GSM1084973 |
Cord Blood Day +3 +4F, +BMSC, Experiment 3 |
| GSM1084974 |
Cord Blood Day 23 +4F -BMSC, Experiment 2 |
| GSM1084975 |
Cord Blood Day 23 +4F -BMSC, Experiment 3 |
| GSM1084976 |
Cord Blood Day 23 +4F +BMSC, Experiment 1 |
| GSM1084977 |
Cord Blood Day 23 +4F +BMSC, Experiment 2 |
| GSM1084978 |
Cord Blood Day 23 +4F +BMSC, Experiment 3 |
| GSM1084979 |
nonviral 4F non-stromal primed cord blood iPSC clone E17C1 p9 |
| GSM1084980 |
nonviral 4F non-stromal primed cord blood iPSC clone E20C2 p18 |
| GSM1084981 |
nonviral 4F non-stromal primed cord blood iPSC clone E24C1 p17 |
| GSM1084982 |
nonviral 4F stromal primed cord blood iPSC clone E5C3 p18 |
| GSM1084983 |
nonviral 4F stromal primed cord blood iPSC clone E12C5 p19 |
| GSM1084984 |
nonviral 4F stromal primed cord blood iPSC clone E17C6 p21 |
| GSM1084985 |
nonviral 7F non-stromal primed cord blood iPSC clone iCB 2-5 p18 |
| GSM1084986 |
nonviral 7F non-stromal primed cord blood iPSC clone iCB8 p19 |
| GSM1084987 |
nonviral 7F non-stromal primed cord blood iPSC clone iCB9 p18 |
| GSM1084988 |
nonviral 7F stromal primed cord blood iPSC clone 6.2 p18 |
| GSM1084989 |
nonviral 7F stromal primed cord blood iPSC clone 6.13 p22 |
| GSM1084990 |
nonviral 7F stromal primed cord blood iPSC clone 19.11 p17 |
| GSM1084991 |
hESC (H7) p38 |
| GSM1084992 |
hESC (H9) p60 |
| GSM1084993 |
hESC (ESO3) p84 |
|
| This SubSeries is part of SuperSeries: |
| GSE44430 |
Comprehensive microarray analysis of reprogramming fidelity in a library of non-integrated induced pluripotent stem cell lines prepared with various derivation methods |
|
| Relations |
| BioProject |
PRJNA189962 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE44425.txt.gz |
373 b |
(ftp)(http) |
TXT |
| GSE44425_non-normalized.txt.gz |
24.4 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...