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Series GSE44433 Query DataSets for GSE44433
Status Public on May 05, 2014
Title Expression data from laser microdissected murine intestinal epithelial cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary Microarray analyses of laser microdissected murine intestinal epithelial cells under the control of maternal inflammation at 17.5 days post conception demonstrates that maternal inflammation differentially regulates 2174 (iWT) and 3345 (iARE) genes in fetal tissue, but these transcriptional profiles were overwritten in the postnatal environment dominated by tissue inflammation at 8 weeks postnatal.
We isolated 50 ng total RNA out of laser microdissected fetal and adult intestinal epithelial cells from the distal ileum and performed microarrays to address whether maternal inflammation programs the fetal intestine towards TNF-driven pathology
 
Overall design Conventionally raised heterozygous TnfΔARE/+ and Tnf+/+ wild type mice on C57BL/6 background were used for this study. Female Tnf+/+ wild type (WT) mice were bred with male TnfΔARE/+ (ARE) mice and vice versa, generating offspring from healthy WT dams (WT and ARE) and inflamed ARE dams (iWT and iARE). On day 17.5 post conception (dpc) and 8 weeks postnatal the fetuses and offspring were sacrificed.The gut was freshly frozen in Optimal Cutting Temperature (O.C.T.) embedding compound (Sakura Finetek, Torrance, USA) on dry ice and stored at -80°C until laser microdissection.
 
Contributor(s) Hemmerling J, Heller K, Hörmannsperger G, Bazanella M, Clavel T, Kollias G, Haller D
Citation(s) 24849654
Submission date Feb 20, 2013
Last update date Aug 04, 2014
Contact name Jana Hemmerling
E-mail(s) Jana.Hemmerling@tum.de
Organization name Technische Universität München
Department ZIEL
Lab Biofunctionality
Street address Gregor-Mendel-Str.2
City Freising
State/province Bavaria
ZIP/Postal code 85350
Country Germany
 
Platforms (1)
GPL16702 NuGO array (mouse) NuGO_Mm1a520177
Samples (40)
GSM1085075 Tnf+/+ fetus from a Tnf+/+ dam at 17.5 dpc, biological rep1
GSM1085076 Tnf+/+ fetus from a Tnf+/+ dam at 17.5 dpc, biological rep2
GSM1085077 Tnf+/+ fetus from a Tnf+/+ dam at 17.5 dpc, biological rep3
Relations
BioProject PRJNA189969

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE44433_RAW.tar 78.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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