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| Status |
Public on Feb 22, 2013 |
| Title |
Pheromone-induced biofilm arrays in the planktonic and pheromone-induced biofilm growth conditions |
| Organism |
Candida albicans |
| Experiment type |
Expression profiling by array
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| Summary |
Candida albicans can stochastically switch between two phenotypes, white and opaque. Opaque cells are the sexually competent form of C. albicans and therefore undergo efficient polarized growth and mating in the presence of pheromone. In contrast, white cells cannot mate, but are induced - under a specialized set of conditions - to form biofilms in response to pheromone. In this work, we compare the genetic regulation of such "pheromone-stimulated" biofilms with that of "conventional" C. albicans biofilms. In particular, we examined a network of six transcriptional regulators (Bcr1, Brg1, Efg1, Tec1, Ndt80, and Rob1) that mediate conventional biofilm formation for their potential roles in pheromone-stimulated biofilm formation. We show that four of the six transcription factors (Bcr1, Brg1, Rob1, and Tec1) promote formation of both conventional and pheromone-stimulated biofilms, indicating they play general roles in cell cohesion and biofilm development. In addition, we identify the master transcriptional regulator of pheromone-stimulated biofilms as C. albicans Cph1, ortholog of Saccharomyces cerevisiae Ste12. Cph1 regulates mating in C. albicans opaque cells, and here we show that Cph1 is also essential for pheromone-stimulated biofilm formation in white cells. In contrast, Cph1 is dispensable for the formation of conventional biofilms. The regulation of pheromone- stimulated biofilm formation was further investigated by transcriptional profiling and genetic analyses. These studies identified 206 genes that are induced by pheromone signaling during biofilm formation. One of these genes, HGC1, is shown to be required for both conventional and pheromone-stimulated biofilm formation. Taken together, these observations compare and contrast the regulation of conventional and pheromone-stimulated biofilm formation in C. albicans, and demonstrate that Cph1 is required for the latter, but not the former.
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| Overall design |
4 condition experiment: white and opaque cells in planktonic and pheromone-induced biofilm conditions with and without alpha pheromone.
WT strain (P37005), the tec1 mutant strain and the cph1 mutant strain
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| Contributor(s) |
Lin C, Bennett RJ |
| Citation(s) |
23637598 |
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| Submission date |
Feb 21, 2013 |
| Last update date |
May 27, 2013 |
| Contact name |
Ching-Hsuan Lin |
| E-mail(s) |
chinghsuanlin@ntu.edu.tw
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| Organization name |
National Taiwan University
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| Department |
Department of Biochemical Science and Technology
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| Street address |
National Taiwan University, 1 Roosevelt Rd. Sec. 4
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| City |
Taipei |
| State/province |
Taiwan |
| ZIP/Postal code |
112 |
| Country |
Taiwan |
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| Platforms (1) |
| GPL16385 |
Agilent-020166 Candida albicans 8 x 15k Custom Expression Array |
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| Samples (17)
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| GSM1085613 |
White cells (P37005) in planktonic growth Replicate 1, 4 hr |
| GSM1085614 |
White cells (P37005) in planktonic growth Replicate 2, 4 hr |
| GSM1085615 |
White cells (tec1) in planktonic growth Replicate 1, 4 hr |
| GSM1085616 |
White cells (tec1) in planktonic growth Replicate 2, 4 hr |
| GSM1085617 |
White cells (cph1) in planktonic growth Replicate 1, 4 hr |
| GSM1085618 |
White cells (cph1) in planktonic growth Replicate 2, 4 hr |
| GSM1085619 |
White cells (P37005) in pheromone-induced biofilm condition Replicate 1, 4 hr |
| GSM1085620 |
White cells (P37005) in pheromone-induced biofilm condition Replicate 2, 4 hr |
| GSM1085621 |
White cells (P37005) in pheromone-induced biofilm condition Replicate 3, 4 hr |
| GSM1085622 |
White cells (tec1) in pheromone-induced biofilm condition Replicate 1, 4 hr |
| GSM1085623 |
White cells (tec1) in pheromone-induced biofilm condition Replicate 2, 4 hr |
| GSM1085624 |
White cells (cph1) in pheromone-induced biofilm condition Replicate 1, 4 hr |
| GSM1085625 |
White cells (wor1) in pheromone-induced biofilm condition Replicate 1, 4 hr |
| GSM1085626 |
White cells (wor1) in pheromone-induced biofilm condition Replicate 2, 4 hr |
| GSM1085627 |
White cells (P37005) in pheromone-induced biofilm condition Replicate 1, 24 hr |
| GSM1085628 |
White cells (wor1) in pheromone-induced biofilm condition Replicate 1, 24 hr |
| GSM1085629 |
Opaque cells (P37005) in pheromone-induced biofilm condition Replicate 1, 4 hr |
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| Relations |
| BioProject |
PRJNA190005 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE44449_RAW.tar |
25.7 Mb |
(http)(custom) |
TAR (of GPR) |
| Processed data included within Sample table |
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