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Series GSE4451 Query DataSets for GSE4451
Status Public on Oct 02, 2006
Title Met Expression Signature in Hepatocytes
Organism Mus musculus
Experiment type Expression profiling by array
Summary To identify HGF/Met regulated genes, we performed expression microarray analysis after inducible activation of Met receptor in primary cultures of hepatocytes established from wild-type control (Alb-Cre +/-) and Met conditional knockout mice (Alb-Cre +/-; Met Fl/Fl).
Keywords: time series design
 
Overall design Total RNA was isolated from untreated hepatocyte cultures as well as from cultures treated with 50 ng/ml of HGF for 0.5, 2, 12 or 24 hours. RNA collected from these experiments was converted to fluorescently labeled cDNA and used for hybridizations of oligonucleotide microarrays. All experiments were repeated in triplicates. Total RNA from pooled wild-type mouse hepatocytes was used as universal reference and all hybridizations were repeated following a reverse flourescing.
 
Citation(s) 16710476
Submission date Mar 13, 2006
Last update date Jan 17, 2013
Contact name Snorri S Thorgeirsson
E-mail(s) snorri_s_thorgeirsson@nih.gov
Phone (301) 496-5688 x204
Fax (301) 496-0734
URL http://neoplasia.nci.nih.gov/
Organization name National Cancer institute/NIH
Department Laboratory of Experimental Carcinogenesis
Lab Cellular and Molecular Biology Section
Street address 37 Convent Dr. # 4146
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platforms (1)
GPL1536 NCI/LMT Mm_FCRF_CGEN oligo
Samples (60)
GSM100050 KO - knock-out Untreated - 0 hrs - repeat 3 - Cy5 - mAdbID:51300
GSM100051 KO - knock-out Treated - 2 hrs - repeat 3 - Cy5 - mAdbID:51302
GSM100052 KO - knock-out Untreated - 0 hrs - repeat 3 - Cy3 - mAdbID:51962
Relations
BioProject PRJNA94725

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