|
Status |
Public on Apr 29, 2014 |
Title |
MiR-155 promotes blood-brain barrier dysfunction in neuroinflammation (part 2) |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
|
Summary |
Over-expression of miR-155 induces changes in the pattern of gene expression of hCMEC/D3 cells. hypothesis tested in the present study was that miR-155 constitute an important regulatory control of the brain endothelial response to inflammatory cytokines.
|
|
|
Overall design |
To identify miR-155 target genes in brain endothelim that might be implicated in BBB dysfunction relevant to human disease, we then analysed changes in mRNA expression of hCMEC/D3 cells that overexpress miR-155 and results were contrasted to cells transfected with scrambled miR. To ectopically express miR-155 in hCMEC/D3 cells, 30 nM of pre-miR-155 and the siPORT Amine transfection agent (Applied Biosystems, Warrington, UK) were combined following the manufacturer’s instructions.
|
|
|
Contributor(s) |
Lopez-Ramirez MA, Wu D, Pryce G, Simpson JE, Reijerkerk A, King-Robson J, Kay O, de Vries HE, Hirst MC, Sharrack B, Baker D, Male DK, Michael GJ, Romero IA |
Citation(s) |
24604078, 27246706 |
|
Submission date |
Feb 26, 2013 |
Last update date |
Oct 03, 2019 |
Contact name |
Miguel Alejandro Lopez-Ramirez |
E-mail(s) |
malopezramirez@ucsd.edu
|
Phone |
858-822-6487
|
Organization name |
University of California, San Diego
|
Department |
School of Medicine
|
Street address |
9500 Gilman Drive
|
City |
La Jolla |
State/province |
California/San Diego |
ZIP/Postal code |
92093 |
Country |
USA |
|
|
Platforms (1) |
GPL6883 |
Illumina HumanRef-8 v3.0 expression beadchip |
|
Samples (6)
|
|
This SubSeries is part of SuperSeries: |
GSE44694 |
MiR-155 promotes blood-brain barrier dysfunction in neuroinflammation |
|
Relations |
BioProject |
PRJNA191031 |