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| Status |
Public on Aug 26, 2013 |
| Title |
Lhx2-regulated genes in hematopoietic stem cell (HSC)-like cells differentiated from mouse embryonic stem (ES) cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We previously demonstrated that hematopoietic stem cell (HSC)-like cells are robustly expanded from mouse embryonic stem (ES) cells by enforced expression of Lhx2, a LIM-homeobox domain (LIM-HD) transcription factor.
Here we established an ES cell line which conditionally expressed Lhx2 by Tet-On system. The ES cells were differentiated into HSC-like cells by Lhx2 expression. Lhx2-regulated genes were identified by comparing the HSC-like cells with those cultured in the absence of Lhx2 expression.
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| Overall design |
Mouse ES with inducible Lhx2 were differentiated on OP9 stromal cells into hematopoietic lineage. On day 5 of the differentiation induction,Lhx2 expression was started by the addition of doxycycline (dox) and the cells were cultured on OP9 stromal cells in the presence of IL-6 and SCF. On day 20, HSC-like cells were harvested and re-seeded onto OP9 stromal cells in the absence of Lhx2 expression by dox-removal for 3 days. these cells were compared with the original HSC-like cells.
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| Contributor(s) |
Kitajima K |
| Citation(s) |
23922318 |
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| Submission date |
Mar 01, 2013 |
| Last update date |
Apr 19, 2018 |
| Contact name |
Satoshi Kondo |
| Organization name |
Toray Industries,Inc.
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| Department |
New Projects Development Division
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| Street address |
Tebiro 6-10-1
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| City |
Kamakura |
| State/province |
Kanagawa |
| ZIP/Postal code |
248-8555 |
| Country |
Japan |
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| Platforms (1) |
| GPL5642 |
3D-Gene Mouse Oligo chip 24k |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA192528 |
