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| Status |
Public on Oct 24, 2014 |
| Title |
Long non-coding RNAs and microRNAs involved in integrated co-regulation of neuronal maturation [microRNA expression] |
| Platform organisms |
Homo sapiens; Mus musculus; Rattus norvegicus; Human alphaherpesvirus 1; Human alphaherpesvirus 2; Human betaherpesvirus 5; Murid betaherpesvirus 1; human gammaherpesvirus 4; JC polyomavirus; Human immunodeficiency virus 1; Murid gammaherpesvirus 4; Human gammaherpesvirus 8; Merkel cell polyomavirus; Mus musculus cytomegalovirus 2; Betapolyomavirus hominis; Betapolyomavirus macacae |
| Sample organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Neurogenesis is a pro-survival process that comprises of dendritic and axonal growth, synaptogenesis, synaptic and neuronal pruning. These complex processes are determined by temporal gene expression during development, which is in turn tightly regulated by long non-coding RNAs and microRNAs. In this study, we investigated the processes implicated in the maturation of primary neuronal cultures based on RNA expression profiling. Correlation between neuron specific gene ontologies of mRNA and non-coding RNAs identified direct regulation of axonogenesis and dendritogenesis. Temporally regulated mRNA and their associated long non-coding RNAs were significantly overrepresented in proliferation and differentiation associated signalling, cell adhesion molecules and neurotrophin signalling pathways during neuronal maturation. Long non-coding RNAs associated with Axin2, Cntn1, Ncam1, Negr1, Ntrk2, Nrxn1 and Sh2b3 displayed an inverse expression profile to their mRNA whereas long non-coding RNA -mRNA pairs for Kit, Prkcb and Ralgds displayed similar expression profiles. These genes were also predicted targets of the altered miRNAs, miR-124, -128, -129-5p, -203, -218, -290-5p, -326, -329, -377 and -495. These microRNAs particularly regulate the cell adhesion molecules, Cntn1, Ncam1, Negr1 and Nrxn1 that determine axonogenesis and dendritogenesis, supporting the observed co-regulation of these biological processes by non-coding RNAs. Verification of expression of these long non-coding RNA-mRNA pairs in an in vitro model of ischemic-reperfusion injury showed an inverse expression profile, thus confirming their role(s) in maintenance of the neuronal structure and function. This neuronal transcriptome (mRNAs, lncRNAs, miRNAs) is in turn orchestrated by C/EBPα/β transcription factors and CTCF, thereby governing intricate control of neuronal development.
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| Overall design |
microRNA expression profiling of maturing primary cortical neurons from E15 mouse embryos. Maturing neurons were harvested on Days 2, 4, 6 and 8.
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| Contributor(s) |
Jeyaseelan K, Armugam A, Sepramaniam S, Kaur P |
| Citation(s) |
25061880 |
| Submission date |
Mar 04, 2013 |
| Last update date |
May 10, 2016 |
| Contact name |
Kandiah Jeyaseelan |
| E-mail(s) |
erijeya@nus.edu.sg
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| Organization name |
National University of Singapore
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| Department |
Biochemistry
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| Street address |
8 Medical Drive
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| City |
Singapore |
| ZIP/Postal code |
117597 |
| Country |
Singapore |
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| Platforms (1) |
| GPL11434 |
miRCURY LNA microRNA Array, 6th generation - hsa, mmu & rno |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE44834 |
Long non-coding RNAs and microRNAs involved in integrated co-regulation of neuronal maturation |
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| Relations |
| BioProject |
PRJNA192569 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE44832_RAW.tar |
8.4 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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