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| Status |
Public on Apr 18, 2013 |
| Title |
Genome-wide maps of chromatin state for HIRA, UBN1 and ASF1a |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The HIRA chaperone complex, comprised of HIRA, UBN1 and CABIN1, collaborates with histone-binding protein ASF1a to incorporate histone variant H3.3 into chromatin in a DNA replication-independent manner. To better understand its function and mechanism, we integrated HIRA, UBN1, ASF1a and histone H3.3 ChIP-seq and gene expression analyses. Most HIRA-binding sites co-localize with UBN1, ASF1a and H3.3 at active promoters and active and weak/poised enhancers. At promoters, binding of HIRA/UBN1/ASF1a correlates with the level of gene expression. HIRA is required for deposition of histone H3.3 at its binding sites. There are marked differences in nucleosome and co-regulator composition at different classes of HIRA-bound regulatory site. Underscoring this, we report novel physical interactions between the HIRA complex and transcription factors, a chromatin insulator and an ATP-dependent chromatin-remodelling complex. Our results map the distribution of the HIRA chaperone across the chromatin landscape and point to different interacting partners at functionally distinct regulatory sites.
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| Overall design |
Examination of 3 histone chaperone proteins in HeLa cells
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| Contributor(s) |
Pchelintsev NA, McBryan T, Adams PD |
| Citation(s) |
23602572 |
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| Submission date |
Mar 11, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Peter D. Adams |
| Organization name |
University of Glasgow, Beatson Institute for Cancer Research
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| Street address |
Switchback Rd, Bearsden
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| City |
Glasgow |
| ZIP/Postal code |
G61 1BD |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE45025 |
Placing the HIRA histone chaperone complex in the chromatin landscape |
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| Relations |
| BioProject |
PRJNA192852 |
| SRA |
SRP019255 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE45024_RAW.tar |
1.3 Gb |
(http)(custom) |
TAR (of BED, BIGWIG, TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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