|
| Status |
Public on Mar 16, 2013 |
| Title |
Amplitude modulation of androgen signaling by c-MYC [ChIP-Seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Androgen-stimulated growth of the molecular apocrine breast cancer is mediated by an androgen receptor (AR)-regulated transcriptional program. Through profiling the genomic licalizations of AR and its co-regulators FOXA1 and TCF7L2 in MDA-MB-453 breast cancer cells, we revealed the molecular details of the AR-centered regulatory network. We further identified that c-MYC is a key downstream target co-regulated by AR, FOXA1 and TCF7L2, and reinforces the transctiopnal activation of androgen-responsive genes in this subtype of breast cancers.
|
| |
|
| Overall design |
AR and FOXA1 ChIP-seq were performed in MDA-MB-453 breast cancer cells with treatment of 5a-dihydrotestosterone (DHT) for 16 h. TCF7L2 ChIP-seq was performed in MDA-MB-453 cells treated with vehicle or DHT for 16 h, respectively. MYC ChIP-seq was performed in MDA-MB-453 cells following 6 h DHT stimulation.
|
| |
|
| Contributor(s) |
Ni M, Chen Y, Brown M |
| Citation(s) |
23530127 |
| |
| Submission date |
Mar 15, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Yiwen Chen |
| E-mail(s) |
ychen26@mdanderson.org
|
| Organization name |
University of Texas MD Anderson Cancer Center
|
| Department |
Bioinformatics and Computational Biology
|
| Lab |
Yiwen Chen
|
| Street address |
1400 Pressler Street
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (6)
|
|
| This SubSeries is part of SuperSeries: |
| GSE45203 |
Amplitude modulation of androgen signaling by c-MYC |
|
| Relations |
| BioProject |
PRJNA193202 |
| SRA |
SRP019497 |
Less...
More...