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| Status |
Public on Oct 01, 2013 |
| Title |
Expression data from knockdown of ZXDC1/2 in PMA-treated U937 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
ZXDC1 augments the expression of various markers of monocyte/macrophage differentiation when over-expressed in the U937 cell line treated with the phorbol ester PMA. Likewise, knockdown of ZXDC1 restricts the induced expression of these markers. We sought to identify specfic gene targets of ZXDC1 during the process of monocyte/macrophage differentiation in U937 by performing gene expression profiling in cells exhibiting reduced expression of ZXDC1 compared to controls.
We used microarray to identify genes regulated by ZXDC during PMA-induced differentiation of U937 monoblasts towards a monocyte/macrophage phenotype
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| Overall design |
An inducible shRNAmir (Tet-On) cell line was established in the U937 background that displayed significant, reproducible, and doxycycline inducible knockdown of ZXDC1. Using this cell line, we created four experimental groups to identify gene targets of ZXDC1 during PMA-induced differention towards a monocyte/macrophage phenotype: Vehicle alone; Vehicle+Doxycycline; PMA alone; PMA+Doxycycline. Each independent group was used for RNA extraction and hybridization on Affymetrix microarray chips.
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| Contributor(s) |
Fontes JD, Ramsey JE |
| Citation(s) |
23954399 |
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| Submission date |
Mar 22, 2013 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Joseph Fontes |
| E-mail(s) |
jfontes@kumc.edu
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| Organization name |
Univ Kansas Medical Center
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| Department |
Biochemistry and Molecular Biology
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| Street address |
3901 Rainbow Blvd
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| City |
Kansas City |
| State/province |
KS |
| ZIP/Postal code |
66160 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (12)
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| GSM1103973 |
Vehicle treated, no doxycycline; biological replicate 1 |
| GSM1103974 |
Vehicle treated, doxycycline treated; biological replicate 1 |
| GSM1103975 |
PMA treated, no doxycycline; biological replicate 1 |
| GSM1103976 |
PMA treated, doxycycline treated; biological replicate 1 |
| GSM1103977 |
Vehicle treated, no doxycycline; biological replicate 2 |
| GSM1103978 |
Vehicle treated, doxycycline treated; biological replicate 2 |
| GSM1103979 |
PMA treated, no doxycycline; biological replicate 2 |
| GSM1103980 |
PMA treated, doxycycline treated; biological replicate 2 |
| GSM1103981 |
Vehicle treated, no doxycycline; biological replicate 3 |
| GSM1103982 |
Vehicle treated, doxycycline treated; biological replicate 3 |
| GSM1103983 |
PMA treated, no doxycycline; biological replicate 3 |
| GSM1103984 |
PMA treated, doxycycline treated; biological replicate 3 |
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| Relations |
| BioProject |
PRJNA193671 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE45417_RAW.tar |
55.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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