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| Status |
Public on Mar 26, 2013 |
| Title |
DNA Methylation data from early human B-cell development |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by array
|
| Summary |
A global DNA methylation and gene expression analysis of early human B-cell development reveals a demethylation signature and transcription factor network. Nucleic Acids Res. 2012 Dec;40(22):11339-51.
We examined DNA methylation and RNA expression status during early B-cell development by sorting multiple replicates of four separate stages of pre-B cells derived from normal human fetal bone marrow and applied high-dimension DNA methylation scanning and expression arrays.
We identified a distinct development-dependent demethylation signature which has gene expression regulatory properties for pre-B cells, and provide a catalog reference for the epigenetic changes that occur in pre-B-cell leukemia and other B-cell-related diseases.
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| Overall design |
Human fetal bone marrow was obtained, and using flow cytometry antibodies, four populations of B-cell developmental stages including stage 1 (S1; predominantly multipotent progenitors before lineage commitment and common lymphoid progenitors), stage II (S2; pre-B-I cells), stage 3 (S3; pre-B-II cells) and stage 4 cells (S4; immature B cells). For 8 indibiduals, DNAs and RNAs were isolated and the methylation and expression changes during early B-cell development were identified using the Illumina HumanMethylation450 Beadchip (Illumina) and GeneChip Human Gene 1.0 ST Array (Affymetrix).
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| Contributor(s) |
Lee S, Xiao Y, Wiemels JL |
| Citation(s) |
23074194 |
| |
| Submission date |
Mar 24, 2013 |
| Last update date |
Mar 22, 2019 |
| Contact name |
Seung-Tae Lee |
| E-mail(s) |
essere2@naver.com
|
| Phone |
+82-2-3410-2710
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| Fax |
+82-2-3410-2719
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| Organization name |
Samsung Medical Center
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| Department |
Laboratory Medicine & Genetics
|
| Street address |
50 Iron-Dong KanangmGu
|
| City |
Seoul |
| State/province |
- None - |
| ZIP/Postal code |
135710 |
| Country |
South Korea |
| |
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| Platforms (1) |
| GPL13534 |
Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482) |
|
| Samples (22)
|
| GSM1104642 |
B-cell precursors at S1_FBM28_S1_DNA methylation |
| GSM1104643 |
B-cell precursors at S1_FBM12_S1_DNA methylation |
| GSM1104644 |
B-cell precursors at S1_FBM16_S1_DNA methylation |
| GSM1104645 |
B-cell precursors at S1_FBM20_S1_DNA methylation |
| GSM1104646 |
B-cell precursors at S1_FBM32_S1_DNA methylation |
| GSM1104647 |
B-cell precursors at S1_FBM24_S1_DNA methylation |
| GSM1104648 |
B-cell precursors at S2_FBM27_S2_DNA methylation |
| GSM1104649 |
B-cell precursors at S2_FBM11_S2_DNA methylation |
| GSM1104650 |
B-cell precursors at S2_FBM15_S2_DNA methylation |
| GSM1104651 |
B-cell precursors at S2_FBM19_S2_DNA methylation |
| GSM1104652 |
B-cell precursors at S2_FBM31_S2_DNA methylation |
| GSM1104653 |
B-cell precursors at S2_FBM23_S2_DNA methylation |
| GSM1104654 |
B-cell precursors at S3_FBM10_S3_DNA methylation |
| GSM1104655 |
B-cell precursors at S3_FBM26_S3_DNA methylation |
| GSM1104656 |
B-cell precursors at S3_FBM18_S3_DNA methylation |
| GSM1104657 |
B-cell precursors at S3_FBM14_S3_DNA methylation |
| GSM1104658 |
B-cell precursors at S3_FBM30_S3_DNA methylation |
| GSM1104659 |
B-cell precursors at S3_FBM22_S3_DNA methylation |
| GSM1104660 |
B-cell precursors at S4_FBM9_S4_DNA methylation |
| GSM1104661 |
B-cell precursors at S4_FBM17_S4_DNA methylation |
| GSM1104662 |
B-cell precursors at S4_FBM21_S4_DNA methylation |
| GSM1104663 |
B-cell precursors at S4_FBM29_S4_DNA methylation |
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| This SubSeries is part of SuperSeries: |
| GSE45461 |
A global DNA methylation and gene expression analysis of early human B-cell development reveals a demethylation signature and transcription factor network. |
|
| Relations |
| BioProject |
PRJNA194408 |
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