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| Status |
Public on Mar 19, 2007 |
| Title |
experiment in midbrain primary cultures, enriched in dopaminergic neurons |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
To identify novel Nurr1 target genes we have used microarrays strategies in rat midbrain primary cultures, enriched in dopaminergic neurons, by the action of basic fibroblast growth factor (bFGF, 20ng/ml) and Sonic hedgedog (SHH), following upregulation of Nurr1 expression by depolarization.To this aim we have treated the cultures after 9 days in vitro for 2h with high KCl and collected 30 min or 2 h after the end of depolarization (2h + 30 min or 2h + 2h). With this experimental protocol we have identify a putative Nurr1 regulator and Nurr1 target
Keywords: depolarization, microarrays, time course.
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| Overall design |
cRNA triplicates of cultures treated for 2h with high KCl and collected 30 min or 2 h after the end of depolarization (2h + 30 min or 2h + 2h) and control was hybridized on triplicate microarrays. The extrapolation of expression values from the images was carried out using different statistical filters and were compared up- and down-regulated
probesets showing significant change between depolarized and control samples
after 30 min or 2 h after the end of depolarization.
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| Contributor(s) |
Volpicelli F, Greco D, Caiazzo M, di Porzio U, Consales C, Perrone Capano C, Colucci D'Amato L |
| Citation(s) |
17506860 |
| Submission date |
Mar 27, 2006 |
| Last update date |
Mar 03, 2017 |
| Contact name |
Umberto di Porzio |
| E-mail(s) |
diporzio@igb.cnr.it
|
| Phone |
+390816132356
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| Fax |
+390816132350
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| Organization name |
Istitute of genetics and biophysics
|
| Department |
neurobiology laboratory
|
| Lab |
di Porzio
|
| Street address |
Via Pietro Castellino 111
|
| City |
Naples |
| ZIP/Postal code |
80131 |
| Country |
Italy |
| |
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| Platforms (1) |
| GPL341 |
[RAE230A] Affymetrix Rat Expression 230A Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA94469 |
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