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| Status |
Public on Aug 16, 2013 |
| Title |
Profiling of glycerol- and CTX-induced models of muscle regeneration in mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Utilizing glycerol and cardiotoxin (CTX) injections in the tibialis anterior muscles of M. musculus provides models of skeletal muscle damages followed by skeletal muscle regeneration. In particular, glycerol-induced muscle regeneration is known to be associated with ectopic adipogenesis. We characterized genome-wide expression profiles of tibialis anterior muscles from wild-type mice injured by either glycerol or CTX injection. Our goal was to detect gene expression changes during the time course of glycerol-induced and CTX-induced muscle regeneration models, that can lead to ectopic adipocyte accumulation.
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| Overall design |
Tibialis anterior muscle of 12 week old wildtype C57BL/6J male mice injected i.m with either glycerol or CTX were collected 3, 7, 14 or 21 days after injection. 6 biological replicates were used for each time points.
Please note that a few replicate samples did not pass QC test, thus, were removed from the submission.
Unchallenged tibialis anterior muscles from a group of 5 wildtype C57BL/6J mice were also as a control. Total RNA was extracted, QCed and hybridized to Affymetrix microarrays.
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| Contributor(s) |
Lukjanenko L, Feige JN, Brachat S |
| Citation(s) |
23976982 |
| |
| Submission date |
Mar 28, 2013 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Sophie Brachat |
| E-mail(s) |
sophie.lemire@novartis.com
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| Organization name |
Novartis Institutes For Biomedical Research
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| Department |
Muskuloskeletal
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| Street address |
Novartis Campus
|
| City |
Basel |
| ZIP/Postal code |
CH-4002 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (48)
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| Relations |
| BioProject |
PRJNA194615 |
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