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| Status |
Public on May 01, 2013 |
| Title |
Mechanisms of tentacle morphogenesis in the sea anemone, Nematostella vectensis |
| Organism |
Nematostella vectensis |
| Experiment type |
Expression profiling by array
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| Summary |
Evolution of the capacity to form secondary outgrowths from the principal embryonic axes was a crucial innovation that potentiated the diversification of animal body plans. Nevertheless, precisely how such outgrowths develop in early-branching metazoan species remains poorly understood. To identify genes potentially involved in tentacle initiation, outgrowth, and maintenance, we performed transcriptional profiling at three different stages of Nematostella development: late planula larvae with tentacle buds, animals with growing tentacles, and mature animals with four-tentacle polyps. At each of these three stages, the animal was bisected such that the oral tentacle portion (head) could be compared to the body column.
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| Overall design |
Tentacle buds, growing tentacles, and mature tentacles, were compared to body columns from each matching stage in duplicate, for a total of 6 samples.
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| Contributor(s) |
Fritz AE, Seidel CW, Ikmi A, Paulson A, Gibson MC |
| Citation(s) |
23633514 |
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| Submission date |
Mar 28, 2013 |
| Last update date |
Jun 06, 2013 |
| Contact name |
Chris W Seidel |
| E-mail(s) |
seidel@phageT4.org
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| Phone |
816 926 9054
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| Organization name |
Stowers Institute
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| Department |
Genomics
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| Lab |
Seidel
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| Street address |
1000 E 50th St
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| City |
Kansas City |
| State/province |
MO |
| ZIP/Postal code |
64110 |
| Country |
USA |
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| Platforms (1) |
| GPL16896 |
Agilent-022085 SIMR Nematostella 44k 11-2008 [Agilent feature number version] |
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| Samples (6)
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| GSM1110189 |
Tentacle buds versus larval body columns, replicate 1 |
| GSM1110190 |
Tentacle buds versus larval body columns, replicate 2 |
| GSM1110191 |
Primary polyp growing tentacles versus body columns, replicate 1 |
| GSM1110192 |
Primary polyp growing tentacles versus body columns, replicate 2 |
| GSM1110193 |
Mature tentacles versus mature body columns, replicate 1 |
| GSM1110194 |
Mature tentacles versus mature body columns, replicate 2 |
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| Relations |
| BioProject |
PRJNA194626 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE45588_RAW.tar |
75.6 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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