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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jun 25, 2013 |
| Title |
FoxO1 Controls Effector to Memory Transition and Maintenance of Functional CD8 T-Cell Memory |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
During a T cell response, naïve CD8 T cells differentiate into effector cells. Subsequently, a subset of effector cells termed memory precursor effector cells (MPECs) further differentiates into functionally mature memory CD8 T cells. The transcriptional network underlying this carefully scripted process is not well understood. Here, we report that the transcription factor FoxO1 plays an integral role in facilitating effector to memory transition and functional maturation of memory CD4 and CD8 T cells. We find that FoxO1 is not required for differentiation of effector cells, but in the absence of FoxO1, memory CD8 T cells displayed features of scenescence and progressive attrition in polyfunctionality, which in turn led to impared recall responses and poor protective immunity. These data suggest that FoxO1 is essential for active maintenance of functional CD8 T cell memory and protective immunity. Under competing conditions in bone marrow
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| Overall design |
Single-cell suspensions from splenocytes of eight samples WT (control) and FoxO1-/- (experimental) LCMV-immune mice were prepared using standard procedures. CD8 T cells were then isoloated using Thy1.2 (CD90.2) (30-H12) microbeads (Miltenyi Biotec). Cells were then stained with anti-CD8, anti-CD44 and Db/NP396 MHC class I tetramer. Activated (CD8+CD44hi), naive (CD8+CD44lo), and virus-specific CD8 T cells were sorted using FACSAria II instrument (BD Biosciences). The purity of the cells was >95%. Total RNA was extracted from the sorted cells by Trizol Reagent. RNA samples were reverse transcribed and Cy3-labeled cDNAs were hyrbidized to Agilent whole Mouse Genome Oligo Microarrays. Fluorscence signals were detected using Agilent's Microarray Scanner system, data was analyzed using the Rosetta Resolver gene expression data analysis system and genes with a fold change < and p-values <0.01 were identified. Microarray data discussed in the paper is focused on virus-specific memory CD8 T cells from samples WT_Tet_2 vs KO_Tet_2.
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| Contributor(s) |
Tejera MM, Kim EH, Sullivan JA, Plisch EH, Suresh M |
| Citation(s) |
23733882 |
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| Submission date |
Apr 01, 2013 |
| Last update date |
Jul 19, 2017 |
| Contact name |
Melba Marie Tejera |
| Organization name |
UW-Madison
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| Department |
Pathobiological Sciences
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| Lab |
M. Suresh
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| Street address |
2015 Linden Drive
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| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53562 |
| Country |
USA |
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| Platforms (1) |
| GPL13912 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA195588 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE45673_RAW.tar |
103.2 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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