Transcriptional profiling of larval stage I - IV of Homarus americanus, for assessment of stage specific developmental gene expression. Individuals from each stage were gathered in July of 2009 and 2010. A reference design was used to permit comparison of all stages. Study aims included assessment of global gene expression of larval development of healthy larvae, and identifying novel molecular pathways involved in H. americanus development.
Overall design
Four condition experiment, stage I - IV individuals: 10 biological replicates per stage. Stages collected independantly from each other; sampling conducted over 2 seasons. Reference sample prepared from pooled RNA of 4 individuals per stage.
Hines DJ, Clark KF, Greenwood SJ. Global gene expression profiling of Homarus americanus (Crustacea) larval stages during development and metamorphosis. Invertebrate Reproduction and Development 2014; 58(2):97-107. doi:10.1080/07924259.2013.834278