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| Status |
Public on Apr 02, 2013 |
| Title |
Regulation by growth temperature of Pseudomonas aeruginosa quorum-sensing depedent virulence factors production involves two RNA-thermometers. |
| Organism |
Pseudomonas aeruginosa |
| Experiment type |
Expression profiling by array
|
| Summary |
Several bacterial human pathogens regulate the production of virulence factors by temperature, expressing them only at 37 °C. Accordingly we show that the production of all P. aeruginosa virulence factors that are dependent on the QS transcriptional regulator RhlR, but only a fraction that are activated by LasR, are induced at 37 °C compared to 30 °C or 25 °C. The RhlR-dependent induction at 37 °C is a posttranscriptional effect due to an RNA thermometer of the ROSE family that thermoregulates the expression of rhlAB operon involved in rhamnolipids production, a virulence associated trait. This RNA structure also affects the expression of the downstream rhlR gene. A second thermometer is present upstream lasI and causes a reduced expression of this gene at lower temperatures without causing a significant decrease of the autoinducer 3-oxo-dodecanoyl homoserine lactone.
Using transcriptomic analysis and quantitative real time PCR we show that these RNA thermometers are the main mechanism of thermoregulation of P. aeruginosa virulence factor production
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| Overall design |
For the transcriptome analysis, cells were growth in PPGAS media at 25 °C and 37 °C, respectively. Samples were harvested at O.D600 of 1.5.
Three biological replicates of each growth condition was used for RNA extraction and hybridization on Affimetrix microarrays.
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| Citation(s) |
25313031 |
| Submission date |
Apr 01, 2013 |
| Last update date |
Jul 06, 2016 |
| Contact name |
Victoria Grosso |
| E-mail(s) |
mvgb28@yahoo.com
|
| Organization name |
Instituto de Investigaciones Biomédicas UNAM
|
| Department |
Biología Molecular y Biotecnología
|
| Street address |
Tercer circuito exterior C.U
|
| City |
México D.F |
| ZIP/Postal code |
04510 |
| Country |
Mexico |
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| Platforms (1) |
| GPL84 |
[Pae_G1a] Affymetrix Pseudomonas aeruginosa Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA195619 |
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