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Status |
Public on Apr 05, 2006 |
Title |
Normal vs. Tumor Prostate miRNA |
Organism |
Homo sapiens |
Experiment type |
Non-coding RNA profiling by array
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Summary |
Comparison of miRNA expression profiles in a small set of prostate needle core biopsies or fine needle aspirates. Keywords: Expression profiling of prostate needle core biopsies
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Overall design |
MicroRNA expression was compared between a pooled normal sample consisting of 10 separate normal adjacent to tumor prostate needle core biopsies, two prostate tumor cell lines (PC3 and LNCaP), two needle core biopsies, and a fine needle aspirate of a prostate tumor metastasis to the supraclavicular lymph node.
MicroRNA was isolated from fresh frozen tissue sections of the needle core biopsies using the mirVana miRNA Isolation kit from Ambion per the manufacturer's instructions. MicroRNA was amplified using 10 ng input and 750 ng of amplified material was subsequently labeled for hybridization. All samples were normalized to the same normal prostate control.
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Contributor(s) |
Mattie MD, Haqq CM |
Citation(s) |
16784538 |
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Submission date |
Mar 29, 2006 |
Last update date |
Mar 16, 2012 |
Contact name |
Michael D. Mattie |
E-mail(s) |
mdmattie@yahoo.com
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Phone |
919-483-5617
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Fax |
919-315-0158
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Organization name |
GlaxoSmithKline
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Department |
Genomics Research
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Lab |
Quantitative Expression
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Street address |
Five Moore Drive
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City |
Research Triangle Park |
State/province |
NC |
ZIP/Postal code |
27709 |
Country |
USA |
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Platforms (1) |
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Samples (6)
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GSM102246 |
Normal prostate vs. needle core tumor |
GSM102247 |
Normal prostate vs. needle core transitional cell metaplasia |
GSM102248 |
Normal prostate vs. Pooled Normal |
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Relations |
BioProject |
PRJNA94411 |