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Series GSE4574 Query DataSets for GSE4574
Status Public on Apr 05, 2006
Title Normal vs. Tumor Prostate miRNA
Organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary Comparison of miRNA expression profiles in a small set of prostate needle core biopsies or fine needle aspirates.
Keywords: Expression profiling of prostate needle core biopsies
 
Overall design MicroRNA expression was compared between a pooled normal sample consisting of 10 separate normal adjacent to tumor prostate needle core biopsies, two prostate tumor cell lines (PC3 and LNCaP), two needle core biopsies, and a fine needle aspirate of a prostate tumor metastasis to the supraclavicular lymph node.

MicroRNA was isolated from fresh frozen tissue sections of the needle core biopsies using the mirVana miRNA Isolation kit from Ambion per the manufacturer's instructions. MicroRNA was amplified using 10 ng input and 750 ng of amplified material was subsequently labeled for hybridization. All samples were normalized to the same normal prostate control.
 
Contributor(s) Mattie MD, Haqq CM
Citation(s) 16784538
Submission date Mar 29, 2006
Last update date Mar 16, 2012
Contact name Michael D. Mattie
E-mail(s) mdmattie@yahoo.com
Phone 919-483-5617
Fax 919-315-0158
Organization name GlaxoSmithKline
Department Genomics Research
Lab Quantitative Expression
Street address Five Moore Drive
City Research Triangle Park
State/province NC
ZIP/Postal code 27709
Country USA
 
Platforms (1)
GPL3238 miRNA microarray
Samples (6)
GSM102113 Normal prostate vs. PC3
GSM102244 Normal prostate vs. LNCaP
GSM102245 Normal prostate vs FNA prostate
Relations
BioProject PRJNA94411

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE4574_RAW.tar 50.0 Kb (http)(custom) TAR (of GPR)

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