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| Status |
Public on Apr 09, 2013 |
| Title |
Expression data from bovine liver |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by array
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| Summary |
Female sex steroid hormones, estradiol-17β (E2) and progesterone (P4) regulate reproductive function and gene expression in a broad range of tissues. Given the central role of the liver in regulating homeostasis including steroid hormone metabolism, we sought to understand how E2-17β and P4 interact to affect global gene expression in liver. Eight ovariectomized cows were randomly assigned to 4 treatment groups applied in a replicated Latin Square design: 1) No hormone supplementation, 2) E2-17β treatment (ear implant), 3) P4 treatment (intravaginal inserts), and 4) E2-17β combined with P4. After 14 d of treatment, liver biopsies were collected, allowing 28 d intervals between periods. Changes in gene expression in the liver biopsies were monitored using Affymetrix bovine-specific arrays. Treatment with E2-17β altered expression of 479 genes, P4 472 genes, and combined treatment significantly altered expression of 468 genes. In total, 578 genes exhibited altered expression including a remarkable number (346 genes) that responded similarly to E2-17β, P4, or combined treatment. Additional evidence for similar gene expression effects of E2-17ß and/or P4 were: principal component analysis placed almost every treatment array at a substantial distance from control arrays; Venn diagrams indicated overall treatment effects for most regulated genes; clustering analysis indicated the two major clusters had all treatments upregulating (cluster 1; 172 genes) or downregulating (cluster 2: 173 genes) expression. Thus, unexpectedly, common biological pathways are regulated by E2-17β and/or P4 in liver. Future studies are needed to elucidate mechanism(s) responsible for overlapping actions of E2-17β and P4 on the liver transcriptome. KEYWORDS: estradiol, progesterone, global gene expression, liver, cows.
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| Overall design |
randomized Latin square design with 4 treatment groups
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| Contributor(s) |
Wiltbank MC, Piccinato CA |
| Citation(s) |
24069207 |
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| Submission date |
Apr 08, 2013 |
| Last update date |
Aug 08, 2019 |
| Contact name |
Carla A Piccinato |
| E-mail(s) |
cpiccinato@uwalumni.com
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| Organization name |
Hospital Albert Einstein
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| Department |
Instituto de Ensino e Pesquisa Albert Einstein
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| Lab |
Centro de Pesquisa Experimental
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| Street address |
Rua Albert Einstein, 627- 2SS Bloco A
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| City |
São Paulo |
| State/province |
SP |
| ZIP/Postal code |
05652-900 |
| Country |
Brazil |
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| Platforms (1) |
| GPL2112 |
[Bovine] Affymetrix Bovine Genome Array |
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| Samples (28)
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| GSM1116700 |
liver control, biological replication 1 |
| GSM1116701 |
liver control, biological replication 2 |
| GSM1116702 |
liver control, biological replication 3 |
| GSM1116703 |
liver control, biological replication 4 |
| GSM1116704 |
liver control, biological replication 5 |
| GSM1116705 |
liver control, biological replication 6 |
| GSM1116706 |
liver control, biological replication 7 |
| GSM1116707 |
liver exposed to estradiol (E2T), biological replication 1 |
| GSM1116708 |
liver exposed to estradiol (E2T), biological replication 2 |
| GSM1116709 |
liver exposed to estradiol (E2T), biological replication 3 |
| GSM1116710 |
liver exposed to estradiol (E2T), biological replication 4 |
| GSM1116711 |
liver exposed to estradiol (E2T), biological replication 5 |
| GSM1116712 |
liver exposed to estradiol (E2T), biological replication 6 |
| GSM1116713 |
liver exposed to estradiol (E2T), biological replication 7 |
| GSM1116714 |
liver exposed to estradiol and progesterone (E2P4T), biological replication 1 |
| GSM1116715 |
liver exposed to estradiol and progesterone (E2P4T), biological replication 2 |
| GSM1116716 |
liver exposed to estradiol and progesterone (E2P4T), biological replication 3 |
| GSM1116717 |
liver exposed to estradiol and progesterone (E2P4T), biological replication 4 |
| GSM1116718 |
liver exposed to estradiol and progesterone (E2P4T), biological replication 5 |
| GSM1116719 |
liver exposed to estradiol and progesterone (E2P4T), biological replication 6 |
| GSM1116720 |
liver exposed to estradiol and progesterone (E2P4T), biological replication 7 |
| GSM1116721 |
liver exposed to progesterone (P4T), biological replication 1 |
| GSM1116722 |
liver exposed to progesterone (P4T), biological replication 2 |
| GSM1116723 |
liver exposed to progesterone (P4T), biological replication 3 |
| GSM1116724 |
liver exposed to progesterone (P4T), biological replication 4 |
| GSM1116725 |
liver exposed to progesterone (P4T), biological replication 5 |
| GSM1116726 |
liver exposed to progesterone (P4T), biological replication 6 |
| GSM1116727 |
liver exposed to progesterone (P4T), biological replication 7 |
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| Relations |
| BioProject |
PRJNA196507 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE45857_RAW.tar |
71.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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