|
| Status |
Public on Apr 07, 2014 |
| Title |
Incorporation of transposable element small RNAs into the Arabidopsis AGO1 protein |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Background: Transposable element 24 nucleotide small RNAs are not efficiently incorporated into the AGO1 protein, which is involved in endogenous RNAi and gene regulation through the microRNA and tasiRNA pathways.
Results: The AGO1 protein incorporates large quantities of transposable element siRNAs when transposable elements are epigenetically activated and transcribed. The incorporation of transposable element siRNAs is at the expense of the most abundant microRNAs. These transposable element siRNAs can act as tasiRNAs, regulating genes that they have partial complementarity to.
Conclusion: Transposable element small RNAs are more dynamic than previously thought. They can be incorporated into AGO1 and regulate genes.
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| |
|
| Overall design |
Three biological replicates of small RNA sequencing from two genotypes
|
| |
|
| Contributor(s) |
Slotkin RK, Panda K |
| Citation(s) |
23863322 |
| |
| Submission date |
Apr 11, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Richard Keith Slotkin |
| E-mail(s) |
slotkin.2@osu.edu
|
| Organization name |
The Ohio State University
|
| Department |
Molecular Genetics
|
| Lab |
Slotkin Laboratory
|
| Street address |
318 West 12th Ave.
|
| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43210 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11221 |
Illumina Genome Analyzer IIx (Arabidopsis thaliana) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA196838 |
| SRA |
SRP020950 |
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